Adeno-Associated Virus Vectors: Design and Delivery

Chapter 1. Stereotactic Intraparenchymal Injection for Gene Delivery to the Brain

Chapter 2. MRI-Guided Intraparenchymal Injection for Gene Delivery to the Brain (Krystof Bankiewicz)

Chapter 3: Intravenous Infusion for Widespread Delivery to Nervous and Peripheral Tissues (Brian Kaspar)

Chapter 4: MRI-Guided Focused Ultrasound for Targeted Gene Delivery to the Brain (Isabelle Aubert)

Chapter 5: Intrathecal Infusion for Gene Delivery to the Nervous System (Mark Tuszynski and myself)

Chapter 6: Direct Intraparenchymal Injection for Gene Delivery to the Spinal Cord

Chapter 7: Retrograde Targeting of Spinal Motor Neurons by Muscle Injection

Chapter 8: Subpial Infusion for Widespread Gene Delivery to the Spinal Cord

Chapter 9: Intraganglionic Microinjection for Gene Delivery to the DRG

Chapter 10: Intravitreal Injection for Gene Delivery to the Eye

Chapter 11: Subretinal Injection for Gene Delivery to the Eye (Luk Vandenberghe)

[Chapters 10 and 11 could be combined into a single chapter for ocular delivery]

Part II: Gene Delivery to Peripheral Tissues

Chapter 12: Intranasal Administration for Gene Delivery to the Lung

Chapter 13: Orotracheal Administration for Gene Delivery to the Lung

Chapter 14: Surgical Tracheal Administration for Gene Delivery to the Lung

[Chapters 13 and 14 could be combined into a single chapter for tracheal delivery]

Chapter 15: Infusion of Coronary Artery for Global Gene Delivery to the Heart

Chapter 16: Aortic Occlusion and Pressurized Infusion for Global Gene Delivery to the Heart

[Chapters 15 and 16 could be combined into a single chapter for global heart delivery]

Chapter 17: Intramyocardial Injection for Targeted Gene Delivery to the Heart

Chapter 18: Intraportal Vein Injection for Global Gene Delivery to the Liver

Chapter 19: Intraparenchymal Injection for Targeted Gene Delivery to the Liver

Chapter 20: Gene Delivery to the Spleen

Chapter 21: Gene Delivery to the Kidney

Chapter 22: Gene Delivery to Skeletal Muscle

Part III: Design of Novel Vectors for Targeting of Specific Tissues and Cell Types

Chapter 23: Design of AAV vectors for Delivery of Large or Multiple Transgenes

Chapter 24: Design of AAV vectors for Delivery of RNAi

Chapter 25: Altering AAV tropism with Directed Evolution

Chapter 27: Altering AAV tropism with Phage Display (Tomas Bjorklund)

Chapter 28: Minipromoters for Tissue- and Cell-Specific Gene Expression (Elizabeth Simpson)