Antibody Engineering: Methods and Protocols

Part I: In Silico Methods

1. Antibody Design and Humanization via In Silico Modeling

Vinodh B. Kurella and Reddy Gali

2. Antibody Affinity Maturation by Computational Design

3. Use of IMGT(R) Databases and Tools for Antibody Engineering and Humanization

Marie-Paule Lefranc, Fran?ois Ehrenmann , Sofia Kossida, V?ronique Giudicelli, and Patrice Duroux

Part II: Generation of Diversity

4. Construction of Human Na?ve Antibody Gene Libraries

Michela Pasello, Alessandra Mallano, Michela Flego, Silvia Zamboni, Anna Maria Giudice, and Katia Scotlandi

5. Construction of Synthetic Antibody Libraries

D?borah Caucheteur, Gautier Robin, Vincent Parez, and Pierre Martineau

6. Construction of Histidine-Enriched Shark IgNAR Variable Domain Antibody Libraries for the Isolation of pH-Sensitive vNAR Fragments

Doreen K?nning, Steffen Hinz, Julius Grzeschik, Christian Schr?ter, Simon Krah, Stefan Zielonka, and Harald Kolmar

7. Display Technologies for Generation of Ig Single Variable Domains

Vladimir Bobkov, Bas van der Woning, and Hans de Haard

8. A Streamlined Approach for the Construction of Large Yeast Surface Display Fab Antibody Libraries

Simon Krah, Julius Grzeschik, Simon Rosowski, Ramona Gaa, Iris Willenbuecher, Deniz Demir, Lars Toleikis, Harald Kolmar, Stefan Becker, and Stefan Zielonka

Part III: Selections of Lead Candidate

9. Phage Display and Selections on Purified Antigens

Magali Colazet and Patrick Chames

10. Selection of Antibodies to Transiently Expressed Membrane Proteins Using Phage Display

Martina L. Jones, Stephen M. Mahler, and Sumukh Kumble

11. Selection of Antibody Fragments against Structured DNA by Phage Display

Mahdi Zeraati, Marcel E. Dinger, and Daniel Christ

12. Selection of Antibody Fragments by Yeast Display

Nathalie Scholler

13. Rapid Selection of High Affinity Antibody scFv Fragments Using Ribosome Display

Birgit Dreier and Andreas Pl?ckthun

14. In Vitro Selection of Single Domain Antibody (VHH) Using cDNA Display

Naoto Nemoto, Shigefumi Kumachi, and Hidenao Arai

15. Sequencing and Affinity Determination of Antigen-Specific B Lymphocytes from Peripheral Blood

Peter Schofield, Rodrigo Vazquez-Lombardi, Mahmoud Abdelatti, Damien Nevoltris, Christopher C. Goodnow, Daniel Christ, and Joanne H. Reed