Astrocytes: Methods and Protocols

Part I: Astrocyte Biology: Overviews and Perspectives

1. The Astrocyte-Neuron Interface: An Overview on Molecular and Cellular Dynamics Controlling Formation and Maintenance of the Tripartite Synapse

Uzma Hasan and Sandeep K. Singh

2. Role of Astrocytes in the Neurogenic Niches

Julia Schneider, Julian Karpf, and Ruth Beckervordersandforth

Part II: Isolation of Astrocytes from Animal Models

3. Isolation and Culture of Astrocytes from Postnatal and Adult Mouse Brains

Yingbo He, Natalie Taylor, and Anindya Bhattacharya

4. Fluorescence-Activated Cell Sorting-Based Isolation and Characterization of Neural Stem Cells from the Adult Zebrafish Telencephalon

Rossella Di Giaimo, Sven Aschenbroich, and Jovica Ninkovic

Part III: Astrocyte Morphology and Functions: Emerging Methods for Their Characterizations In Vivo, In Situ, and In Vitro

5. A Method to Visualize the Nanoscopic Morphology of Astrocytes In Vitro and In Situ

Janosch P. Heller and Dmitri A. Rusakov

6. A Specialized Method to Resolve Fine 3D Features of Astrocytes in Non-Human Primate (Marmoset, Callithrix Jacchus) and Human Fixed Brain Samples

Ga?l Quesseveur, Aymeric Fouquier d'H?rou?l, Keith K. Murai, and David S. Bouvier

7. Cisterna Magna Injection in Rats to Study Glymphatic Function

Marta Ramos, Nicholas Burdon Bechet, Roberta Battistella, Chiara Pavan, Anna L.R. Xavier, Maiken Nedergaard, and Iben Lundgaard

8. Preparing the Astrocyte Perivascular Endfeet Transcriptome to Investigate Astrocyte Molecular Regulations at the Brain Vascular Interface

Anne-C?cile Boulay, No?mie Mazar?, Bruno Saubam?a, and Martine Cohen-Salmon

9. Monitoring Interneuron-Astrocyte Signaling and Its Consequences on Synaptic Transmission

Sara Mederos and Gertrudis Perea

10. Astrocytes and Circadian Rhythms: An Emerging Astrocyte-Neuron Synergy in the Timekeeping System

Olga Barca Mayo, Luca Berdondini, and Davide De Pietri Tonelli

11. In Vitro Engulfment Assay to Measure Phagocytic Activity of Astrocytes Using Synaptosomes

Youkyeong Gloria Byun and Won-Suk Chung

12. Quantifying Compartment-Specific Protein Translocation in Astrocytes by Object-Oriented Image Analysis: Mitochondrial Translocation of PKCδ

Kiavasch M.N. Farid and Amin Derouiche

13. Imaging of Local and Global Sodium Signals in Astrocytes

Niklas J. Gerkau, Karl W. Kafitz, and Christine R. Rose

14. Detection of Protein Uptake in In Vitro Cultured Astrocytes Exemplified by the Uptake of the Serine Protease, Tissue Plasminogen Activator