Autophagy in Differentiation and Tissue Maintenance: Methods and Protocols

1. Visualization and Measurement of Multiple Components of the Autophagy Flux

Tracey Evans, Robert Button, Oleg Anichtchik, and Shouqing Luo

2. Mitochondrial Redox Sensor for Drosophila Female Germline Stem Cells

Kiran Suhas Nilangekar and Bhupendra V. Shravage

3. Induction and Detection of Autophagy in Aged Hematopoietic Stem Cells by Exposing Them to Microvesicles Secreted by HSC-Supportive Mesenchymal Stromal Cells

Rohan S. Kulkarni, Manmohan S. Bajaj, and Vaijayanti P. Kale

4. Skeletal Muscle Lysosomal Function via Cathepsin Activity Measurement

Kristyn Gumpper, Matthew Sermersheim, Michael X. Zhu, and Pei-Hui Lin

5. Autophagy in Adipocyte Differentiation

Zhipeng Tao, Longhua Liu, Louise D. Zheng, and Zhiyong Cheng

6. Assessing Autophagy in the Leydig Cells

Hui Gao, Chao Liu, and Wei Li

7. Determination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells

Sinem Tun?er and Sreeparna Banerjee

8. Simultaneous Detection of Autophagy and Epithelial to Mesenchymal Transition in the Non-Small Cell Lung Cancer Cells

Javad Alizadeh, Shahla Shojaei, Adel Sepanjnia, Mohammad Hashemi, Eftekhar Eftekharpour, and Saeid Ghavami

9. Autophagy in Zebrafish Extraocular Muscle Regeneration

Alfonso Saera-Vila, Phillip E. Kish, and Alon Kahana

10. The Detection Techniques for Autophagy-Associated Cell Death Related Genes and Proteins: Gene Expression Assay and Immunohistochemistry

Melek Ozturk, Ozlem Dagdeviren Ozsoylemez, and Fatma Kaya Dagistanli

11. Cloning of Autophagy-Related MicroRNAs

Deniz Gulfem Ozturk, Muhammed Kocak, and Devrim Gozuacik

12. Assays to Monitor Aggrephagy in Drosophila Brain

Anne-Claire Jacomin and Ioannis P. Nezis

13. Methods for Monitoring Autophagy in Silkworm Organs

Aurora Montali, Morena Casartelli, Daniele Bruno, Annalisa Grimaldi, and Gianluca Tettamanti

14. Immunofluorescence Staining Protocols for Major Autophagy Proteins Including LC3, P62, ULK1 in Mammalian Cells in Response to Normoxia and Hypoxia

Wen Li, Shupeng Li, Yifang Li, Xiaoying Lin, Yongquan Hu, Tian Meng, Baojin Wu, Rongrong He, and Du Feng

15. Identification of Novel Autophagy Inhibitors via Cell-Based High-Content Screening

Georgios Konstantinidis, Sonja Sievers, and Yao-Wen Wu

16. Porcine Cell-Free System to Study Mammalian Sperm Mitophagy

Won-Hee Song and Peter S