Biomedical Nanotechnology: Methods and Protocols

Preface...
Table of Contents...
Contributing Authors...

1. Quantification of siRNA Duplexes Bound to Gold Nanoparticle Surfaces
Jilian R. Melamed, Rachel S. Riley, Danielle M. Valcourt, Margaret M. Billingsley, Nicole L. Kreuzberger, and Emily S. Day

2. Ligand Exchange and 1H NMR Quantification of Single- and Mixed-Moiety Thiolated Ligand Shells on Gold Nanoparticles
Ashley M. Smith and Jill E. Millstone

3. Nanoparticle Tracking Analysis for Determination of Hydrodynamic Diameter, Concentration, and Zeta Potential of Polyplex Nanoparticles
David R. Wilson and Jordan J. Green

4. Magnetic Characterization of Iron Oxide Nanoparticles for Biomedical Applications
Lorena Maldonado-Camargo, Mythreyi Unni, and Carlos Rinaldi

5. Preparation of Magnetic Nanoparticles for Biomedical Applications
Xiaolian Sun and Shouheng Sun

6. Brain-Penetrating Nanoparticles for Analysis of the Brain Microenvironment
Elizabeth Nance

7. Volumetric Bar-Chart Chips for Biosensing
Yujun Song, Ying Li, and Lidong Qin

8. qFlow Cytometry-Based Receptoromic Screening: A High-Throughput Quantification Approach Informing Biomarker Selection and Nanosensor Development
Si Chen, Jared Weddell, Pavan Gupta, Grace Conard, James Parkin, and Princess I. Imoukhuede

9. Evaluating Nanoparticle Binding to Blood Compartment Immune Cells in High-Throughput with Flow Cytometry
Shann S. Yu

10. A Gold@Polydopamine Core-Shell Nanoprobe for Long-Term Intracellular Detection of MicroRNAs in Differentiating Stem Cells
Chun Kit K. Choi, Chung Hang J. Choi, and Liming Bian

11. Antibody-Conjugated Single Quantum Dot Tracking of Membrane Neurotransmitter Transporters in Primary Neuronal Cultures
Danielle M. Bailey, Oleg Kovtuna, and Sandra J. Rosenthal

12. Spectroscopic Photoacoustic Imaging of Gold Nanorods
Austin Van Namen and Geoffrey P. Luke

13. Dual Wavelength-Triggered Gold Nanorods for Anti-Cancer Treatment
Dennis B. Pacardo, Frances S. Ligler, and Zhen Gu

14. Photolabile Self-Immolative DNA-Drug Nanostructures
Xuyu Tan and Ke Zhang

15. Enzyme-Responsive Nanoparticles for the Treatment of Disease
Cassandra E. Callmann and Nathan C. Gianneschi

16. NanoScript: A Versatile Nanoparticle-Based Synthetic Transcription Factor for Innovative Gene Manipulation
Kholud Dardir, Christopher Rathnam, and KiBum Lee

17. Glucose-Responsive Insulin Delivery by Microneedle-Array Patches Loaded with Hypoxia-Sensitive Vesicles
Jicheng Yu, Yuqi Zhang, and Zhen Gu

18. Electrospun Nanofiber Scaffolds and their Hydrogel Composites for the Engineering and Regeneration of Soft Tissues
Ohan S. Manoukian, Rita Matta, Justin Letendre, Paige Collins, Augustus D. Mazzocca, and Sangamesh G. Kumbar

19. Application of Hydrogel Template Strategy in Ocular Drug Delivery
Crystal S. Shin, Daniela C. Marcano, Kinam Park, and Ghanashyam Acharya

20. High-Accuracy Determination of Cytotoxic Responses from Graphene Oxide Exposure using Imaging Flow Cytometry
Sandra Vranic and Kostas Kostarelos

21. Air-Liquid Interface Cell Exposures to Nanoparticle Aerosols
Nastassja A. Lewinski, Nathan J. Liu, Akrivi Asimakopoulou, Eleni Papaioannou, Athanasios Konstandopoulos, and Michael Riediker

22. Returning to the Patent Landscapes for Nanotechnology: Assessing the Garden That It Has Grown Into
Diana M. Bowman, Douglas J. Sylvester, and Anthony D. Marino