Bone Research Protocols

Part I: Culture of Osteoblasts and Osteocytes.- 1. Primary Human Osteoblast Cultures.- Jane P. Dillon, Victoria J. Waring-Green, Adam M. Taylor, Peter Wilson, Mark Birch, Alison Gartland, James A. Gallagher .- 2. Osteoblast Isolation from Murine Calvaria and Long Bones.- Jenneke Klein-Nulend and Astrid D. Bakker .- 3. Rat Osteoblast Cultures.- Isabel R. Orriss, Sarah E.B. Taylor and Timothy R. Arnett.- 4. Isolation of Primary Avian Osteocytes.- Cor M. Semeins, Astrid D. Bakker, Jenneke Klein-Nulend.- 5. Isolation of Mouse Osteocytes using Cell Fractionation for Gene Expression Analysis. .- Christine Halleux, Ina Kramer, Cyril Allard and Michaela Kneissel .- 6. Studying Osteocyte Function using the Cell Lines MLO-Y4 and MLO-A5.- Jennifer Rosser and Lynda F. Bonewald.- 7. Isolation, Differentiation and Characterisation of Human Bone Marrow Stem Cells in vitro and in vivo.- Rahul S. Tare, Peter D. Mitchell, Janos Kanczler, Richard O.C. Oreffo.- Part II: Culture of Osteoclasts.- 8. Rodent Osteoclast Cultures.- Isabel R. Orris and Timothy R. Arnett.- 9. Isolation and Culture of Primary Chicken Osteoclasts.- Patricia Collin-Osdoby and Phillip Osdoby.- 10. Isolation and Purification of Rabbit Osteoclasts .- Fraser P. Coxon, Michael J. Rogers and Julie C. Crockett.- 11. Generation of Human Osteoclasts from Peripheral Blood.- Kim Henriksen, Morten A. Karsdal, Adam Taylor, Denise Tosh and Fraser P. Coxon.- 12. Osteoclast Formation in Mouse Co-Cultures.- Cecile Itzstein and Robert J. van't Hof.- 13. Rankl-Mediated Osteoclast Formation From Murine RAW 264.7 Cells.- Patricia Collin-Osdoby and Phillip Osdoby.- Part III: Biochemical and Molecular Analysis of Bone Cells.- 14. Transfection of Osteoclasts and Osteoclast Precursors.- Julie C. Crockett, David J. Mellis and Adam Taylor.- 15. Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation.- Aymen I. Idris.- 16. Analysis of Transcriptional Regulation in Bone Cells .- Huilin Jin and Stuart Ralston.- 17. Extraction of Nucleic Acids from Bone.- Alun Hughes, Tracey L. Steward and Val Mann.- 18. Analysis of Gene Expression in Bone by Quantitative RT/PCR.- Alun Hughes.- Part IV: Microscopial Techniques.- 19. Histomorphometry in Rodents .- Reinhold G. Erben and Martin Gl?sman.- 20. Studying Gene Expression in Bone by in situ Hybridization .- Ina Kramer, Rishard Sali, Mira Susa, and Michaela Kneissel.- 21. Immunostaining of Skeletal Tissue .- Tobias Kurth and Cosimo De Bari.- 22. Techniques for the Study of Apoptosis in Bone.- Sudeh Riahi and Brendon Noble .- 23. Transmission Electron Microscopy of Bone.- Vincent Everts, Anneke Niehof, Wikky Tigchelarr-Gutter and Wouter Beertsen.- 24. Scanning Electron Microscopy of Bone.- Alan Boyde.- 25. Fluorescence Imaging of Osteoclasts using Confocal Microscopy.- Fraser P. Coxon.- 26. Live Imaging of Bone Cell and Organ Cultures .- Sarah L. Dallas and Pat A. Veno.- Part V: Imaging TEchniques .- 27. Analysis of Bone Architecture in Rodents using Micro Computed Tomography .- Rob van't Hof.- 28. Bone Measurments by Peripheral Quantitatvie Computer Tomography in Rodents .- J?rg Gasser, and Johannes Willnecker.- 29. Quantitative X-Ray Imaging of Rodent Bone by Faxitron.- JH Duncan Basset, Anne van der Spek, Apostolos Gogakos, Graham R Williams.- 30. Bioluminescence Imaging of Bone Metastasis in Rodents .- Thomas J.A. Snoeks, Ermond van Beek, Ivo Que, Eric L. Kaijzel and Clemens W.G.M. L?wik.- 31. Fourier Transform Infrared Imaging of Bone .- Elefterios P. Paschalis.- 32. Raman Microscopy of Bone.- Simon R. Goodyear and Richard M. Aspden.- Part VI: In Vivo Techniques.- 33. The Calvarial Injection Assay.- Robert J. van't Hof.- 34. Ovariectomy/Orchidectomy in Rodents.- Aymen I. Idris.- Part VIII: Mechanical Loading Techniques.- 35. Mechanical Properties of Bone Ex Vivo .- Simon R. Goodyear and Rchiard M. Aspden.- 36. Mechanical Stimulation of Bone Cells using Fluid Flow.- Carmen Huesa and Astrid D. Bakker.- 37. Using Cell and Organ Cultur