Brassinosteroids: Methods and Protocols

1. Protocol for Extraction and Isolation of Brassinosteroids from Plant Tissues

Danuse Tarkowsk? and Miroslav Strnad

2. Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye

Johan M. Winne, Niloufer G. Irani, Jos Van den Begin, and Annemieke Madder

3. Physiological Analysis of Brassinosteroid Responses and Sensitivity in Rice

Hongning Tong and Chengcai Chu

4. Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability in Darkness

Claudia Corval?n and Sunghwa Choe

5. Approaches to Study Light Effects on Brassinosteroid Sensitivity

Sandi Paulisic, Maria Jos? Molina-Contreras, Irma Roig-Villanova, and Jaime F. Mart?nez-Garc?a

6. A Technical Framework for Studying the Signaling Nexus of Brassinosteroids and Immunity

Rosa Lozano-Dur?n and Youssef Belkhadir

7. Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing

Trevor Nolan, Sanzhen Liu, Hongqing Guo, Lei Li, Patrick Schnable, and Yanhai Yin

8. Quantitation of Cell Type-Specific Responses to Brassinosteroid by Deep Sequencing of Polysome-Associated Polyadenylated RNA

Kristina Vragovic, Elizabeth Bartom, and Sigal Savaldi-Goldstein

9. Methods for Modeling Brassinosteroid-Mediated Signaling in Plant Development

10. Quantitative Microscopic Analysis of Plasma Membrane Receptor Dynamics in Living Plant Cells

Yu Luo and Eugenia Russinova

11. Analysis of In Vitro DNA Interactions of Brassinosteroid-Controlled Transcription Factors Using Electrophoretic Mobility Shift Assay

Simon J. Unterholzner, Wilfried Rozhon, and Brigitte Poppenberger

12. Identification of Brassinosteroid Signaling Complexes by Coimmunoprecipitation and Mass Spectrometry

Walter van Dongen, Luc van Heerde, Sjef Boeren, and Sacco C. de Vries

13. Simplified Enrichment of Plasma Membrane Proteins from Arabidopsis thaliana Seedlings Using Differential Centrifugation and Brij-58 Treatment

Carina A. Collins, Michelle E. Leslie, Scott C. Peck, and Antje Heese

14. Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation

15. The Primary Root of Sorghum bicolor (L. Moench) as a Model System to Study Brassinosteroid Signaling in Crops

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