Cdna Libraries: Methods and Applications

Part I: Enhanced Approaches to Inherent Problems

1. Isolation of Fungal Infection Structures from Plant Tissue by Flow Cytometry for Cell-Specific Transcriptome Analysis

Hiroyuki Takahara, Elmar Endl, and Richard O'Connell

2. Preparation of a High Quality cDNA Library from a Single-Cell Quantity of mRNA Using Chum-RNA

Hiroshi Nojima and Takahiro Tougan

3. Construction of a Full-Length cDNA Library from Castor Endosperm for High-throughput Functional Screening

Chaofu Lu, James G. Wallis, and John Browse

4. Full-Length Transcriptome Analysis Using a Bias-Free cDNA Library Prepared with the Vector-Capping Method

Seishi Kato, Mio Oshikawa, and Kuniyo Ohtoko

5. Construction of Improved Yeast Two-Hybrid Libraries

Richard H. Maier, Christina J. Maier, and Kamil ?nder

6. Normalization of Full-Length Enriched cDNA

Ekaterina A. Bogdanova, Ekaterina V. Barsova, Irina A. Shagina, Alexander Scheglov, Veronika Anisimova, Laura L. Vagner, Sergey A. Lukyanov, and Dmitry A. Shagin

7. Bioinformatic Methods for Finding Differentially Expressed Genes in cDNA Libraries, Applied to the Identification of Tumour Vascular Targets

John M.J. Herbert, Dov J. Stekel, Manuela Mura, Michail Sychev, and Roy Bicknell

Part II: Visionary Applications

8. Enzymatic Production of RNAi Libraries from cDNAs and High-Throughput Selection of Effective shRNA Expression Constructs

Kohtaroh Sugao and Kenzo Hirose

9. Construction of Small RNA cDNA Libraries for High-Throughput Sequencing

Cheng Lu and Vikas Shedge

10. Focusing Mutations Within Random Libraries to Distinct Areas: Protein Domain Library Generation by Overlap Extension (PDLGO)

Andreas Gratz and Joachim Jose

11. Generation of Families of Construct Variants Using Golden Gate Shuffling

Carola Engler and Sylvestre Marillonnet

12. Application of Full-Length cDNA Resources to Gain-of-Function Technology for Characterization of Plant Gene Function

Youichi Kondou, Mieko Higuchi, Takanari Ichikawa, and Minami Matsui

13. Construction of Yeast Surface-Displayed cDNA Libraries

Scott Bidlingmaier and Bin Liu

14. Identification of Protein/Target Molecule Interactions Using Yeast Surface-Displayed cDNA Libraries

Scott Bidlingmaier and Bin Liu

15. SNP Discovery by Transcriptome Pyrosequencing

W. Brad Barbazuk and Patrick S. Schnable

16. RNA-seq Analysis of Gene Expression and Alternative Splicing by Double-Random Priming Strategy

Michael T. Lovci, Hai-Ri Li, Xiang-dong Fu, and Gene W. Yeo

17. Generation of a Large Catalog of Unique Transcripts for Whole-Genome Expression Analysis in Non-Model Species

Diana Bellin, Alberto Ferrarini, and Massimo Delledonne