Cell-Based Microarrays: Methods and Protocols

1. Cell-Based Microarrays: Overview Ella Palmer 2. Cell-Based Co-Transfection Microarrays for Use with HEK293T Cells on a Poly D-Lysine Polystyrene Coated Microplate Meenal Soni and Fang Lai 3. Large-Scale Cell-Based Microarrays and Their Use with HEK293T Cells and Downstream Apoptotic Assays Ella Palmer and Tom C. Freeman 4. A Novel Fluorescent Transcriptional Reporter for Cell-Based Microarray Assays Tanya M. Redmond and Michael D. Uhler 5. High-Throughput Subcellular Protein Localization Using Transfected-Cell Arrays Yuhui Hu and Michal Janitz 6. Cell Arrays for the Measurement of Organelle Dynamics in Living Cells Holger Erfle, Tautvydas Lisauskas, Christoph Claas, J?rgen Reymann, and Vytaute Starkuviene 7. High-Throughput Immunofluorescence Microscopy Using Yeast Spheroplast Cell-Based Microarrays Wei Niu, G. Traver Hart, and Edward M. Marcotte 8. Cell-Based Microarrays of Infectious Adenovirus Encoding Short Hairpin RNA (shRNA) Hansj?rgen Volkmer and Frank Weise 9. Reverse Transfected Cell Microarrays in Infectious Disease Research Andreas Konrad, Ramona Jochmann, Elisabeth Kuhn, Elisabeth Naschberger, Priya Chudasama, and Michael St?rzl 10. Transfected Cell Microarrays for the Expression of Membrane-Displayed Single-Chain Antibodies Baochuan Lin and James B. Delehanty 11. Blood Cell Capture on Antibody Micro-Arrays and Monitoring of the Cell Capture Using Surface Plasmon Resonance Imaging Yoann Roupioz, Sarah Milgram, Andr? Roget, and Thierry Livache 12. Immobilized Culture and Transfection Microarray of Nonadherent Cells Satoshi Yamaguchi, Erika Matsunuma, and Teruyuki Nagamune 13. Plasma Polymer and PEG-Based Coatings for DNA, Protein, and Cell Microarrays Andrew L. Hook, Nicolas H. Voelcker, and Helmut Thissen 14. Polymer Microarrays for Cellular High-Content Screening Salvatore Pernagallo and Juan J. Diaz-Mochon 15. High-Throughput Analyses of Gene Functions on a Cell Chip by Electroporation Koichi Kato and Hiroo Iwata 16. Microfluidic Image Cytometry Ken-ichiro Kamei, Jing Sun, Hsian-Rong Tseng, and Robert Damoiseaux