Cell Imaging Techniques: Methods and Protocols

1. Digital Images are Data - and Should be Treated as Such

Douglas W. Cromey

2. Epi-fluorescence Microscopy

Donna J. Webb and Claire M. Brown

3. Live-Cell Migration and Adhesion Turnover Assays

Lacoste, J. Young, K. and Brown, C. M.

4. Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of HemostaticProteins in Human Venous Valves

Winifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill

5. Colocalization Analysis in Fluorescence Microscopy

Jeremy Adler

6. A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown

Sunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman

7. Light Sheet Microscopy in Cell Biology

Raju Tomer, Khaled Khairy and Philipp J. Keller

8. Image-based High-throughput Screening for Inhibitors of Angiogenesis

Lasse Evensen, Wolfgang Link, and James B. Lorens

9. Intravital Microscopy to Image Membrane Trafficking in Live Rats

Andrius Masedunskas, Monika Sramkova, Laura Parente and Roberto Weigert

10. Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential Interference Contrast Microscopy and Planar Illumination Microscopy

Wei Sun, Lehui Xiao, and Ning Fang

11. Laser Scanning Cytometry. Principles and Applications: An Update

Piotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz

12. Laser Capture Microdissection for Protein and NanoString RNA Analysis

Yelena Golubeva, Rosalba Salcedo, Claudius Mueller, Lance A. Liotta, and Virginia Espina

13. Viewing Dynamic Interactions of Proteins and a Model Lipid Membrane with Atomic Force Microscopy

Anthony S. Quinn, Jacob H. Rand, Xiao-Xuan Wu, and Douglas J. Taatjes

14. Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy

Luda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko

15. Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation

Joanna L. MacKay and Sanjay Kumar

16. Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells

Lilia A. Chtcheglova and Peter Hinterdorfer

17. Porosome: The Secretory NanoMachine in Cells

18. Stereology and Morphometry of Lung Tissue

Christian M?hlfeld, Lars Knudsen, Matthias Ochs

19. A Novel Combined Imaging/Stereological Method for the Analysis of Human Sural Nerve Biopsies for Clinical Diagnosis

Michele A. VonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes

20. Correlative Light-Electron Microscopy as a Tool to Study In vivo Dynamics and uUtrastructure of Intracellular Structures

Elena V. Polishchuk, Roman S. Polishchuk and Alberto Luini

21. Photooxidation Technology for Correlative Light and Electron Microscopy

Claudia Meisslitzer-Ruppitsch, Clemens R?hrl, Carmen Ranftler, Herbert Stangl, Josef Neum?ller, Margit Pavelka, Adolf Ellinger

22. Electron Microscopy of Endocytic Pathways

Carmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neum?ller, Margit Pavelka

23. Morphological Analysis of Autophagy

Keisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori

24. Cytochemical Detection of Peroxisomes and Mitochondria

Nina A. Bonekamp, Markus Islinger, Maria G?mez L?zaro, and Michael Schrader

25. Histochemical Detection of Lipid Droplets in Cultured Cells

Michitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto

26. Environmental Scanning Electron Microscopy in Cell Biology

J. E. McGregor, L. T. L. Staniewicz, S. E. Guthrie (ne? Kirk) and