Cytochrome P450 Protocols

1. Bioluminescent Assays for Cytochrome P450 Enzymes

Douglas S. Auld, Henrike Veith, and James J. Cali

2. In Vitro Drug-Drug Interaction Assay

Michael Zientek and Kuresh Youdim

3. High-Throughput Mass Spectrometric Cytochrome P450 Inhibition Screening

Kheng B. Lim, Can C. Ozbal, and Daniel B. Kassel

4. The Synthesis, Characterization and Application of 13C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites

Christopher McCullough, Phani Kumar Pullela, Sang-Choul Im, Lucy Waskell, Daniel Sem

5. High-Throughput Fluorescence Assay for Cytochrome P450 Mechanism-Based Inactivators

Cesar Kenaan, Haoming Zhang, and Paul F. Hollenberg

6. Identification of Endogenous Substrates of Orphan Cytochrome P450 Enzymes Through the Use of Untargeted Metabolomics Approaches

Qian Cheng and F. Peter. Guengerich

7. Genetic and Mass Spectrometric Tools for Elucidating the Physiological Function(s) of Cytochrome P450 Enzymes from Mycobacterium Tuberculosis

Hugues Ouellet, Eric D. Chow, Shenheng Guan, Jeffery S. Cox, Alma L. Burlingame, and Paul R. Ortiz de Montellano

8. An Escherichia coli Expression-Based Approach for Porphyrin Substitution in Heme Proteins

Michael B. Winter, Joshua J. Woodward, and Michael A. Marletta

9. Expression in Escherichia coli of a Cytochrome P450 Enzyme with a Cobalt Protoporphyrin IX Prosthetic Group

Wesley E. Straub, Clinton R. Nishida, and Paul R. Ortiz de Montellano

10. Nanodiscs in the Studies of Membrane-Bound Cytochrome P450 Enzymes

A. Luthra, M. Gregory, Y.V. Grinkova, I.G. Denisov, and S.G. Sligar

11. Rapid LC-MS Drug Metabolite Profiling Using Bioreactor Particles

Linlin Zhao, Besnik Bajrami, and James F. Rusling

12. Fluorescence-Based Screening of cytochrome P450 activities in Intact Cells

M. Teresa Donato and M. Jos? G?mez-Lech?n

13. Screening for Cytochrome P450 Reactivity with a Reporter Enzyme

Kersten S. Rabe and Christof M. Niemeyer

14. High-Throughput Fluorescence Assay of Cytochrome P450 3A4

Qian Cheng and F. Peter. Guengerich

15. Targeted Protein Capture for Analysis of Electrophile-Protein Adducts

Rebecca E. Connor, Simona G. Codreanu, Lawrence J. Marnett, and Daniel C. Liebler

16. DNA Shuffling of Cytochrome P450 Enzymes

James B.Y.H. Behrendorff, Wayne A. Johnston, and Elizabeth M.J. Gillam

17. Measurement of P450 Difference Spectra Using Intact Cells

Wayne A. Johnston and Elizabeth M.J. Gillam

18. DNA Shuffling of Cytochromes P450 for Indigoid Pigment Production

Nedeljka N. Rosic

19. P450 oxidoreductase - Genotyping, Expression, Purification of Recombinant Protein and Activity Assessments of Wild-Type and Mutant Protein

Vishal Agrawal and Walter L. Miller

20. LICRED: A Versatile Drop-In Vector for Rapid Generation of Redox-Self-Sufficient Cytochromes P450

Federico Sabbadin, Gideon G. Grogan and Neil C. Bruce

21. Update on Allele Nomenclature for Human Cytochromes P450 and the Human Cytochrome P450 Allele (CYP-allele) Nomenclature Database

Sarah C. Sim and Magnus Ingelman-Sundberg

22. Simultaneous in vivo Phenotyping of CYP Enzymes

Sussan Ghassabian and Michael Murray

23. Detection of Regulatory Polymorphisms: High-Throughput Capillary DNase-I Footprinting

Matthew Ha