Detection and Typing Strategies for Pathogenic Escherichia Coli

Chapter 1 Introduction to Pathogenic Escherichia coli.- 1.1 Pathotypes of Escherichia coli causing diarrhoeal diseases.- 1.2 Escherichia coli.- 1.3 Pathogenic types of E. coli.- 1.4 Shiga toxin-producing and Enterohemorrhagic E. coli.- 1.4.1 Virulence determinants of Shiga-toxin producing E. coli.- 1.4.2 Shiga-toxin producing E. coli-mediated disease.- 1.4.3 Epidemiology of Shiga toxin-producing E. coli.- 1.4.4 Ecology of Shiga-toxin producing E. coli.- 1.4.5 Transmission of Shiga-toxin producing E. coli.- 1.4.6 Shiga-toxin producing E. coli occurrence in foods.- 1.5 Enterotoxigenic E. coli.- 1.6 Enteropathogenic E. coli.- 1.7 Enteroinvasive E. coli.- 1.8 Enteroaggregative and Diffusely Adherent E. coli..-1.9 References.- Chapter 2 Isolation and detection of pathogenic Escherichia coli in foods.- 2.1 Introduction.- 2.2 General method of isolation for E. coli.- 2.3 Shiga toxin-producing E. coli.- 2.3.1 Culture and isolation of Shiga toxin-producing E. coli.- 2.3.2 Molecular detection of Shiga toxin-producing E. coli.- 2.4 Enumeration of Shiga toxin-producing E.coli.- 2.5 Immunological detection methods for pathogenic E. coli.- 2.6 Cell culture assays used for pathogenic E. coli.- 2.6.1 Cell cytotoxicity assay for Shiga toxin-producing E. coli.- 2.6.2 Cell adherence assays.- 2.7 Enteroinvasive E. coli.- 2.8 Enterotoxigenic E. coli.- 2.9 Enteropathogenic E. coli.- 2.10 Enteroaggregative E. coli and Diffusely Adherent E. coli.- 2.11 References.- Chapter 3 Typing and Subtyping methods for pathogenic Escherichia coli.- 3.1 Introduction.- 3.2 Biochemical profiling.- 3.3 Serotyping.- 3.4 Phage typing.- 3.5 Multilocus enzyme electrophoresis.- 3.6 Multilocus sequence typing.- 3.7 Pulsed-field gel electrophoresis.- 3.8 Multiple-locus variable number tandem repeat analysis.- 3.9 Repetitive element palindromic polymerase chain reaction.- 3.10 Random amplified polymorphic DNA.- 3.11 Shiga toxin subtypes and bacteriophage insertion sites.- 3.12 Lineage specific polymorphism analysis.- 3.13 Whole genome sequencing.- 3.14.- References.- Chapter 4 Emerging and future trends and technologies for the detection and typing of Escherichia coli.- 4.1 Future trends in foodborne illness and E. coli.- 4.2 Future trends in the detection and typing of pathogens.- 4.2.1 Microarrays.- 4.2.2 Matrix Assisted Laser-Desorption Ionization-Time-Of-Flight Mass Spectrometry.- 4.2.3 Nanotechnology.- 4.2.4 Next-generating sequencing of the whole bacterial genome.- 4.3 References