Fibrosis: Methods and Protocols

Preface...
Table of Contents...
Contributing Authors...

Part I: Perspective

1. Human Fibrotic Diseases: Current Challenges in Fibrosis Research
Joel Rosenbloom, Edward Macarak, Sonsoles Piera-Velazquez, and Sergio A. Jimenez

Part II: Animal Models of Fibrosis 2. The Bleomycin Model of Pulmonary Fibrosis
Tianju Liu, Francina Gonzalez De Los Santos, and Sem H. Phan

3. Intra-Dermal Injections of Bleomycin to Model Skin Fibrosis
Toshiyuki Yamamoto

4. Assessing the Effects of Fibrosis on Lung Function by Light Microscopy-Coupled Stereology
Bartosz Pilecki and Grith Lykke Sorensen

5. Transplanting Human Skin Grafts onto Nude Mice to Model Skin Scars
Jie Ding and Edward E. Tredget

6. Hypertrophic Scarring in the Rabbit Ear: A Practical Model for Studying Dermal Fibrosis
Layla Nabai and Aziz Ghahary

7. Mouse and Rat Models of Induction of Hepatic Fibrosis and Assessment of Portal Hypertension
Sabine Klein, Robert Schierwagen, Frank Erhard Uschner, and Jonel Trebicka

8. Mouse Models of Corneal Scarring
Laure Ritti?, Audrey E.K. Hutcheon, and James D. Zieske

9. Modeling Cardiac Fibrosis in Mice: (Myo)Fibroblast Phenotype after Ischemia
Daniela Fraccarollo, Paolo Galuppo, and Johann Bauersachs

10. Characterization of Mesenchymal-Fibroblast Cells using the Col1a2 Promoter/Enhancer
Ian M. H. Li, Amy L. Horwell, Grace Chu, Benoit de Crombrugghe, and Goerge Bou-Gharious

Part III: Cell Culture Models

11. Isolation and Culture of Primary Murine Hepatic Stellate Cells
Sabine Weiskirchen, Carmen G. Tag, Sibille Sauer-Lehnen, Frank Tacke, and Ralf Weiskirchen

12. Isolation and Culture of Adipose-Derived Stromal Cells from Sub-Cutaneous Fat
Lawrence J. Liew, Huan Ting Ong, and Rodney J. Dilley

13. Isolation of Live Fibroblasts by Fluorescence-Activated Cell Sorting
Tripp Leavitt, Michael S. Hu, and Michael T. Longaker

14. Detection of Infiltrating Mast Cells using a Modified Toluidine Blue Staining
Nahum Puebla-Osorio, Seri N. E. Sarchio, Stephen E. Ullrich, and Scott N. Byrne

15. Cell-Populated Collagen Lattice Models
Beate Eckes, Fang Wang, Laure Ritti?, Gabriele Scherr, and Paola Zigrino

16. Traction Force Measurement using Deformable Microposts
Tianfa Xie, Jamar Hawkins, and Yubing Sun

17. Mechanical Deformation of Cultured Cells with Hydrogels
Christal A. Worthen, Laure Ritti?, and Gary J. Fisher

18. Preparation of Decellularized Lung Matrices for Cell Culture and Protein Analysis
Franziska E. Uhl, Darcy E. Wagner, and Daniel J. Weiss

Part IV: ECM Proteins -- Purification, Quantification, and Analysis

19. Type I Collagen Purification from Rat Tail Tendons
Laure Ritti?

20. Purification of Human Plasma/Cellular Fibronectin and Fibronectin Fragments
Giampiero Pietrocola, Simonetta Rindi, Giulia Nobile, and Pietro Speziale

21. Laser Capture Microdissection of Tissue Sections for High-Throughput RNA Analysis
Julia Hoffmann, Jochen Wilhelm, and Grazyna Kwapiszewska

22. Collagen Quantification in Tissue S