Handbook of Elispot: Methods and Protocols

Part I: Setting and Performing ELISPOT Assay

1. Unique Strengths of ELISPOT for T Cell Diagnostics

Paul V. Lehmann and Wenji Zhang

2. The Impact of Harmonization on ELISPOT Assay Performance

7. ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes

Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny

8. ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells

Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny

9. ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion

Jun-Seop Shin and Chung-Gyu Park

10. Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell

Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny

Part IV. Image and Data Analysis

11. How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells' Secretory Activity

Sylvia Janetzki and Cedrik M. Britten

Part II: ELISPOT for Veterinary Research

3. Equine ELISPOT Assay to Study Secretion of IFNγ and IL-4 from Peripheral Blood Mononuclear Cells

7. ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes

Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny

8. ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells

Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny

9. ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion

Jun-Seop Shin and Chung-Gyu Park

10. Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell

Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny

Part IV. Image and Data Analysis

11. How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells' Secretory Activity

Jodi Hagen, Chris Hartnett, J.P. Houchins, Steeve Gigu?re, and Alexander E. Kalyuzhny

4. Utilization of Feline ELISPOT for Mapping Vaccine Epitopes

7. ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes

Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny

8. ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells

Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny

9. ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion

Jun-Seop Shin and Chung-Gyu Park

10. Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell

Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny

Part IV. Image and Data Analysis

11. How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells' Secretory Activity

Jeffrey R. Abbott, Ruiyu Pu, James K. Coleman, and Janet K. Yamamoto

5. Analyzing Cellular Immunity to AAV in a Canine Model Using ELISPOT Assay

7. ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes

Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny

8. ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells

Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny

9.