Heterologous Gene Expression in E.Coli: Methods and Protocols

1. Adjustment of Codon Usage Frequencies by Codon Harmonization Improves Protein Expression and Folding Evelina Angov, Patricia M. Legler, and Ryan M. Mease 2. SUMO Fusion Technology for Enhanced Protein Expression and Purification in Prokaryotes and Eukaryotes Yilin Yan, James Strickler, and Tauseef Butt 3. Molecular and Chemical Chaperones for Improving the Yields of Soluble Recombinant Proteins Ario de Marco 4. Genetic Selection of Solubility-Enhanced Proteins Using the Twin-Arginine Translocation System Adam C. Fisher, Mark A. Rocco, and Matthew P. DeLisa 5. Protein Folding Liquid Chromatography Quan Bai and Xindu Geng 6. Site-Specific Protein Labeling by Intein-Mediated Protein Ligation Inca Ghosh, Nancy Considine, Elissa Maunus, Luo Sun, Aihua Zhang, John Buswell, Thomas C. Evans, Jr., and Ming-Qun Xu 7. Efficient Expression of Human Aromatase (CYP19) in E. coli Norio Kagawa 8. Expression of Recombinant Cytochromes c in E. coli Yuri Y. Londer 9. Semi-Synthesis of Glycoproteins from E. coli through Native Chemical Ligation Jonathan P. Richardson and Derek Macmillan 10. Expression of Recombinant Proteins with Uniform N-Termini Orsolya Kir?ly, Lan Guan, and Mikl?s Sahin-T?th 11. Recent Developments in Difficult Protein Expression: A Guide to E. coli Strains, Promoters, and Relevant Host Mutations James C. Samuelson 12. Periplasmic Chaperones Used to Enhance Functional Secretion of Proteins in E. coli Martin Schlapschy and Arne Skerra 13. Engineering Unusual Amino Acids into Peptides Using Lantibiotic Synthetase Jun-ichi Nagao, Kouki Shioya, Yoshitaka Harada, Ken-ichi Okuda, Takeshi Zendo, Jiro Nakayama, and Kenji Sonomoto 14. The Targeted Expression of Nucleotide Sugar Transporters to the E. coli Inner Membrane Joe Tiralongo and Andrea Maggioni 15. Detection of Protein-Protein Interactions in Bacteria by GFP-Fragment Reconstitution Akira Kanno, Takeaki Ozawa, and Yoshio Umezawa 16. Enhancing the Solubility of Recombinant Proteins in Escherichia coli by Using Hexahistidine-Tagged Maltose-Binding Protein as a Fusion Partner Ping Sun, Joseph E. Tropea, and David S. Waugh 17. Introducing Predetermined Mutations throughout a Target Gene Using TDEM (Transposon Directed Base-Exchange Mutagenesis) Yun Cheol Kim 18. Fluorescent Site-Specific Labeling of Escherichia coli Expressed Proteins with Sfp Phosphopantetheinyl Transferase Aihua Zhang, Luo Sun, John Buswell, Nancy Considine, Inca Ghosh, Anastasiya Masharina, Christopher Noren, and Ming-Qun Xu