High Content Screening: A Powerful Approach to Systems Cell Biology and Phenotypic Drug Discovery

Part I: Reagents and Kits for Measuring Cells and their Phenotypes

1. Applications and Caveats on the Utilization of DNA-specific Probes in Cell-based Assays

Roy Edward

2. General Staining and Segmentation Procedures for High Content Imaging and Analysis

Kevin M Chambers, Bhaskar S Mandavilli, Nick J Dolman and Michael S Janes

3. Tools to Measure Cell Health and Cytotoxicity Using High Content Imaging and Analysis

Bhaskar S Mandavilli, Robert J Aggeler, and Kevin Chambers

4. Cell-based High Content Analysis of Cell Proliferation and Apoptosis

Bhaskar S Mandavilli, Michelle Yann and Scott Clarke

5. Tools to Measure Autophagy using High Content Imaging and Analysis

Nick J Dolman, Brent A. Samson, Kevin M. Chambers, Michael S Janes, and Bhaskar S Mandavilli

Part II: Selecting Microtiter Plates, Identify Imaging Artifacts, Rnai Specificity, Data Handling, Kinetic Assays, and Ultra-High Throughput HCS

6. Guidelines for Microplate Selection in High Content Imaging

O.J. Trask

7. Quality Control for High-Throughput Imaging Experiments using Machine Learning in CellProfiler

Mark-Anthony Bray and Anne E. Carpenter

8. High Content Screening Approaches That Minimize Confounding Factors in RNAi, CRISPR and Small Molecule Screening

Steven A. Haney

9. Strategies and solutions to Maintain and Retain Data from High Content Imaging, Analysis, and Screening Assays

Karol Kozak, Bernt Rinn, Oliver Leven, and M. Emmenlauer

10. Live-Cell High Content Screening in Drug Development

Milan Esner, Felix Meyenhofer, and Marc Bickle

11. Challenges and Opportunities in Enabling High-Throughput, Miniaturized High-Content Screening

Debra Nickischer, Lisa Elkin, Normand Cloutier, Jonathan O'Connell, Martyn Banks, and Andrea Weston

Part III: Harnessing Translocation, Signaling Pathways, Single Cell Analysis, and Multidimensional Data for Phenotypic Analysis

12. Translocation Biosensors - Versatile Tools To Probe Protein Functions In Living Cells

Verena Fetz, Roland H. Stauber, and Shirley K. Knauer

13. High Content Positional Biosensor Assay to Screen for Compounds that Prevent or Disrupt Androgen Receptor and Transcription Intermediary Factor 2 Protein-Protein Interactions

Yun Hua, Daniel P. Camarco, Christopher J. Strock, and Paul A. Johnston

14. High Content Imaging Assays for IL-6-induced STAT3 Pathway Activation in Head and Neck Cancer Cell Lines

Paul A. Johnston, Malabika Sen, Yun Hua, Daniel Camarco, Tong Ying Shun, John S. Lazo, and Jennifer R. Grandis

15. Single Cell and Population Level Analysis of HCA Data

David Novo, Kaya Ghosh, and Sean Burke

16. Utilization of Multidimensional Data in the Analysis of Ultra High Throughput High Content Phenotypic Screens

Judith Wardwell-Swanson and Yanhua Hu

Section IV: Phenotypic Models - Primary Neurons, Neural Stem/Progenitor Cells, Induced-Pluripotent Stem Cells, Multicellular Tumor Spheroids, Endothelial/Mesenchymal Stem Cell Co-Cultures, and Zebrafish Embryos

17. High Content Screening of Mammalian Primary Cortical Neurons

Dario Motti, Murray Blackmore, John Bixby, and Vance Lemmon

18. Human Derived Neurons and Neural Progenitor Cells in High Content Imaging Applications

Joshua A. Harrill

19. Determination of Hepatotoxicity in iPSC-Derived Hepatocytes by Multiplexed High-Content Assays

Oksana Sirenko and Evan F. Cromwell

20. The Generation of Three-Dimensional Head and Neck Cancer Models for Drug Discovery in 384-well Ultra-Low Attachment Microplates

David A. Close