High-Throughput Rnai Screening: Methods and Protocols
(Book #1470 in the Methods in Molecular Biology Series)
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1. Establishing an Infrastructure for High-Throughput Short-Interfering RNA Screening
Hongwei Yin, Chris Sereduk, and Nanyun Tang
2. Optimization of Transfection Conditions for siRNA Screening
Justin J. Montoya and David O. Azorsa
Carina Von Schantz-Fant and Jani Saarela 4. Genome-Wide siRNA Screening Using Forward Transfection: Identification of Modulators of Membrane Trafficking in Mammalian Cells
Mariana G. Bexiga and Jeremy C. Simpson 5. Pooled shRNA Screening in Mammalian Cells as a Functional Genomic Discovery Platform
Katarzyna Jastrzebski, Bastiaan Evers, and Roderick L. Beijersbergen 6. A Protocol for a High-Throughput Multiplex Cell Viability Assay
Daniel F. Gilbert and Michael Boutros 7. RNAi Screening of Leukemia Cells Using Electroporation
Anupriya Agarwal and Jeffrey W. Tyner 8. Self-Assembled Cell Microarray (SAMcell) for High-Throughput RNAi Screening
Hanshuo Zhang and Juan Li 9. In Vitro Pooled shRNA Screening to Identify Determinants of Radiosensitivity
Alessandro Ceroni, Geoff S. Higgins, and Daniel V. Ebner 10. Three-Dimensional Spheroid Cell Culture Model for Target Identification Utilizing High-Throughput RNAi Screens
Lakesla R. Iles and Geoffrey Bartholomeusz 11. In Vitro High Throughput RNAi Screening to Accelerate the Process of Target Identification and Drug Development
Hongwei Yin and Michelle Kassner 12. High-Throughput, Liquid-Based Genome-Wide RNAi Screening in C. elegans
Linda P. O'Reilly, Ryan R. Knoerdel, Gary A. Silverman, and Stephen C. Pak 13. Design and Methods of Large-Scale RNA Interference Screens in Drosophila
Jia Zhou and Chao Tong 14. Genome-Wide RNAi Screening in C. elegans for Aging and Innate Immunity
Amit Sinha and Robbie Rae 15. RNAi-Assisted Genome Evolution (RAGE) in Saccharomyces cerevisiae
Tong Si and Huimin Zhao 16. RNAi Screening in Spodoptera frugiperda
Subhanita Ghosh, Gatikrushna Singh, Bindiya Sachdev, Ajit Kumar, Pawan Malhotra, Sunil K. Mukherjee, and Raj K. Bhatnagar 17. Morphology and Gene Expression Screening with Morpholinos in Zebrafish Embryos
Li-Chuan Tseng, Chih-Hao Tang and Yun-Jin Jiang 18. Live Cell Microscopy-Based RNAi Screening in the Moss Physcomitrella patens
Tomohiro Miki, Yuki Nakaoka, and Gohta Goshima
19. Data Analysis For High Throughput RNAi Screening
David O. Azorsa, Megan A. Turnidge and Shilpi Arora
Format:Paperback
Language:English
ISBN:1493981684
ISBN13:9781493981687
Release Date:June 2018
Publisher:Humana
Length:262 Pages
Weight:1.06 lbs.
Dimensions:0.6" x 7.0" x 10.0"
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