Host-Pathogen Interactions: Methods and Protocols

1. Genetic Association Studies in Host-Pathogens Interaction Analysis

Jose Luis Royo and Luis Miguel Real

2. Bacterial Genotyping Methods: From the Basics to Modern

Margarita M. Ochoa-D?az, Silvana Daza-Giovannetty, and Doris G?mez-Camargo

Part I: Animal-Pathogen Interaction Section

3. Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens

Gili Aviv and Ohad Gal-Mor

4. The Usage of a Bioluminescence Reporter System to Image Promoter Activity During Host Infection

Gili Aviv and Ohad Gal-Mor

5. lacZ Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens

Gili Aviv and Ohad Gal-Mor

6. Western Blotting Against Tagged Virulence Determinants to Study Bacterial Pathogenicity

Gili Aviv and Ohad Gal-Mor

7. Molecular Methods to Analyze the Effect of Proteins Expressed by Salmonella During Its Intracellular Stage

Carlos Medina, Beatriz Mesa-Pereira, Eva Ma Camacho, Amando Flores, and Eduardo Santero

8. Organoids as a Model to Study Infectious Disease

Kristen A. Engevik, Andrea L. Matthis, Marshall H. Montrose, and Eitaro Aihara

9. Surface Proteome Biotinylation Combined with Bioinformatic Tools as a Strategy for Predicting Pathogen Interacting Proteins

Anita Horvatic, Josipa Kules, Nicolas Guillemin, Franjo Martinkovic, Iva Stimac, Vladimir Mrljak, and Mangesh Bhide

10. Systems Biology Modeling to Study Pathogen-Host Interactions

M?berra Fatma Cesur and Saliha Durmuş

11. Phage Therapy: Various Perspectives on How to Improve the Art

Stephen T. Abedon

Part II: Microbe-Microbe Interaction Section

12. Application of RNA-seq and Bioimaging Methods to Study Microbe-Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression

Cristina Isabel Amador, Claus Sternberg, and Lars Jelsbak

13. Serial Dilution-Based Growth Curves and Growth Curve Synchronization for High-Resolution Time Series of Bacterial Biofilm Growth

Fernando Govantes

14. Detection of Bacterial Quorum Sensing Molecules

Elke Stein and Adam Schikora

Part III: Plant-Pathogen Interaction Section

15. Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae

Jos? S. Rufi?n, Diego L?pez-M?rquez, Nieves L?pez-Pag?n, Murray Grant, Javier Ruiz-Albert, and Carmen R. Beuz?n

16. Introduction of Genetic Material in Ralstonia solanacearum through Natural Transformation and Conjugation

Anthony Perrier, Patrick Barberis, and Stephane Genin

17. In Vitro and In Vivo Secretion/Translocation Assays to Identify Novel Ralstonia solanacearum Type 3 Effectors

Fabien Lonjon, Nemo Peeters, St?phane Genin, and Fabienne Vailleau

18. Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains

19. Methods to Quantify Biotic-Induced Stress in Plants

Marcel Bach-Pages and Ga