Human T-Lymphotropic Viruses: Methods and Protocols

Part I: Epidemiology and Transmission

1. Serological and Molecular Methods to Study Epidemiological Aspects of Human T-Cell Lymphotropic Virus Type 1 Infection

Olivier Cassar and Antoine Gessain

2. Molecular Epidemiology Database for Sequence Management and Data Mining

Thessika Hiala Almeida Ara?jo, Filipe Ferreira de Almeida Rego, and Luiz Carlos Junior Alcantara

3. Reporter Systems to Study HTLV-1 Transmission

4. Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Infection Using Co-Culture with Jurkat LTR-Luciferase or Jurkat LTR-GFP Reporter Cells

Sandrine Alais, H?l?ne Dutartre, and Renaud Mahieux

5. Isolation of Exosomes from HTLV-Infected Cells

Robert A. Barclay, Michelle L. Pleet, Yao Akpamagbo, Kinza Noor, Allison Mathiesen, and Fatah Kashanchi

Part II: Promoter Activity of HTLVs' Proteins

6. A Luciferase Functional Quantitative Assay for Measuring NF-ĸB Promoter Transactivation Mediated by HTLV-1 and HTLV-2 Tax Proteins

Elisa Bergamo, Erika Diani, Umberto Bertazzoni, and Maria Grazia Romanelli

7. Generation of a Tet-On Expression System to Study Transactivation Ability of Tax-2

Fabio Bignami, Riccardo Alessio Sozzi, Umberto Bertazzoni, and Elisabetta Pilotti

8. EGF Uptake and Degradation Assay to Determine the Effect of HTLV Regulatory Proteins on the ESCRT-Dependent MVB Pathway

Colin Murphy and Noreen Sheehy

Part III: Genotyping and Gene Expression

9. Methods for Identifying and Examining HTLV-1 HBZ Post-Translational Modifications

Jacob Al-Saleem, Mamuka Kvaratskhelia, and Patrick L. Green

10. High-Throughput Mapping and Clonal Quantification of Retroviral Integration Sites

11. STR Profiling of HTLV-1-Infected Cell Lines

Vittoria Raimondi, Sonia Minuzzo, Vincenzo Ciminale, and Donna M. D'Agostino

12. Expression of HTLV-1 Genes in T-cells Using RNA Electroporation

Mariangela Manicone, Francesca Rende, Ilaria Cavallari, Andrea K. Thoma-Kress, and Vincenzo Ciminale

Part IV: Cellular Dynamics

13. Quantification of Cell Turnover in the Bovine Leukemia Virus Model

Alix de Brogniez, Pierre-Yves Barez, Alexandre Carpentier, Geronimo Gutierrez, Michal Reichert, Karina Trono, and Luc Willems

14. Analysis of NK Cell Function and Receptor Expression During HTLV-1 and HTLV-2 Infection

Federica Bozzano, Francesco Marras, and Andrea De Maria

Part V: Therapy

15. Overview of Targeted Therapies for Adult T-Cell Leukemia/Lymphoma