Kinase Inhibitors: Methods and Protocols

1. Targeting Cancer with Small Molecular Weight Kinase Inhibitors

Doriano Fabbro, Sandra W. Cowan-Jacob, Henrik M?bitz, and Georg Martiny-Baron

2. Small Molecule Protein and Lipid Kinase Inhibitors in Inflammation and Specific Models for their Evaluation

Matthias Gaestel and Alexey Kotlyarov

3. Measuring the Activity of Leucine Rich Repeat Kinase 2 (LRRK2) - A Kinase Involved in Parkinson's Disease

Byoung Dae Lee, Xiaojie Li, Ted M. Dawson, and Valina L. Dawson

4. Measuring PI3K Lipid Kinase Activity

Elisa Ciraolo, Alessia Perino, and Emilio Hirsch

5. A Fluorescence Polarization Assay for the Discovery of Inhibitors of the Polo-box Domain of Polo-like Kinase 1

Wolfgang Reindl, Klaus Strebhardt, and Thorsten Berg

6. Assessment of Hepatotoxicity Potential of Drug Candidate Molecules Including Kinase Inhibitors by Hepatocyte Imaging Assay Technology and Bile Flux Imaging Assay Technology

Jinghai J. Xu, Margaret C. Dunn, Arthur R. Smith, and Eric S. Tien

7. Kinase Inhibitor Selectivity Profiling using Differential Scanning Fluorimetry

Oleg Fedorov, Frank H. Niesen, and Stefan Knapp

8. Chemoproteomic Characterization of Protein Kinase Inhibitors using Immobilized ATP

James S. Duncan, Timothy A.J. Haystead, and David W. Litchfield

9. Proteome-wide identification of staurosporine-binding kinases using Capture Compound Mass Spectrometry

Jenny J. Fischer, Olivia Y. Graebner ne? Baessler, and Mathias Dreger

10. Affinity Purification of Proteins Binding to Kinase Inhibitors Immobilised on Self Assembling Monolayers

Marcus Bantscheff, Scott Hobson, and Bernhard Kuster

11. Kinase Inhibitor Profiling using Chemoproteomics

Markus Schirle, Eugene C. Petrella, Scott M. Brittain, David Schwalb, Edmund Harrington, Ivan Cornella-Taracido, and John A. Tallarico

12. Covalent Crosslinking of Kinases with Their Corresponding Peptide Substrates

Alexander V. Statsuk and Kevan M. Shokat

13. Receptor Tyrosine Kinase Inhibitor Profiling using Bead-based Multiplex Sandwich Immunoassays

Oliver P?tz, Nicole Schneiderhan-Marra, Tanja Henzler, Thomas Herget, and Thomas O. Joos

14. Monitoring Phosphoproteomic Response To Targeted Kinase Inhibitors Using Reverse-Phase Protein Microarrays

Gabriela Lavezzari and Mark R. Lackner

15. Measuring Phosphorylation Specific Changes in Response to Kinase Inhibitors in Mammalian Cells Using Quantitative Proteomics

Nurhan ?zl?, Marc Kirchner, and Judith Jebanathirajah Steen

16. Investigation of Acquired Resistance to EGFR-Targeted Therapies in Lung Cancer Using cDNA Microarrays

Kian Kani, Rafaella Sordella, and Parag Mallick