Lipid-Activated Nuclear Receptors: Methods and Protocols

1. Quantification of Oxysterol Nuclear Receptor Ligands by LC/MS/MS

Lilia Magomedova and Carolyn L. Cummins

2. A Stable Luciferase Reporter System to Characterise LXR Regulation by Oxysterols and Novel Ligands

Samantha A. Hutchinson and James L. Thorne

3. Isolation and Analysis of Human Monocytes and Adipose Tissue Macrophages

Jean-Baptiste Julla, Raphaelle Ballaire, Tina Ejlalmanesh, Jean-Fran?ois Gautier, Nicolas Venteclef, and Fawaz Alzaid

4. Glucan Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages

Emelie Barreby, Andr? Sulen, and Myriam Aouadi

5. Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity

6. Bone Marrow-Derived Macrophage Immortalization of LXR Nuclear Receptor-Deficient Cells

Ana Ram?n-V?zquez, Juan Vladimir de la Rosa, Carlos Tabraue, and Antonio Castrillo

7. Dual Crosslinking Chromatin-Immunoprecipitation Protocol for Next Generation Sequencing (ChIPseq) in Macrophages

David A. Rollins and Inez Rogatsky

8. Analysis of LXR Nuclear Receptor Cistrome through ChIP-seq Data Bioinformatics

Juan Vladimir de la Rosa, Ana Ram?n-V?zquez, Carlos Tabraue, and Antonio Castrillo

9. Quantifying Cellular Cholesterol Efflux

Sabrina Robichaud and Mireille Ouimet

10. Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection

Estibaliz Glar?a, Jonathan Matalonga, and Annabel F. Valledor

11. Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency

Matthew C. Gage

12. Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution

Ada Weinstock and Edward A. Fisher

13. Preparation of Frozen Liver Tissues for Integrated Omics Analysis

Ning Liang, Rongrong Fan, Saioa Goni, and Eckardt Treuter

14. Identification of Nuclear Receptor Targets By Chromatin Immunoprecipitation in Fatty Liver

Natalia Becares and In?s Pineda-Torra

15. The LPS/D-Galactosamine-Induced Fulminant Hepatitis Model to Assess the Role of Ligand-Activated Nuclear Receptors on the NLRP3 Inflammasome Pathway In Vivo

Yasmine Sebti, Lise Ferri, Mathilde Zecchin, Justine Beauchamp, Denis Mogilenko, Bart Staels, H?l?ne Duez, and Benoit Pourcet

16. Analyzing T-Cell Plasma Membrane Lipids by Flow Cytometry

Kirsty E. Waddington, In?s Pineda-Torra