Lymphoma: Methods and Protocols

1. Origin and Pathogenesis of B Cell Lymphomas

Marc Seifert, Ren? Scholtysik, and Ralf K?ppers

2. Flow Cytometry for Non-Hodgkin and Classical Hodgkin Lymphoma

David Wu, Brent L. Wood, and Jonathan R. Fromm

3. Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells

Ralf K?ppers, Markus Schneider, and Martin-Leo Hansmann

4. PCR-Based Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes by GeneScan Analysis or Heteroduplex Analysis for Clonality Assessment in Lymphoma Diagnostics

Elke Boone, Brenda Verhaaf, and Anton W. Langerak, on behalf of the EuroClonality / BIOMED-2 Collaborative Study Group

5. Expression Cloning of Human B Cell Immunoglobulins

Hedda Wardemann and Juliane Kofer

6. Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR

Menno C. van Zelm, Magdalena A. Berkowska, and Jacques J.M. van Dongen

7. The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies

Martin J.S. Dyer

8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas

Nikos Darzentas and Kostas Stamatopoulos

9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies

Sebastian B?ttcher, Matthias Ritgen, and Michael Kneba

10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR

Christiane Pott, Monika Br?ggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba

11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation

Miriam Sonnet, Constance B?r, Michael Rehli, Dieter Weichenhan, and Christoph Plass

12. Gene Expression Profile Analysis of Lymphomas

Katia Basso and Ulf Klein

13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas

Maciej Giefing and Reiner Siebert

14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens

Vu N. Ngo

15. Studying MicroRNAs in Lymphoma

Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg

16. Molecular Methods of Virus Detection in Lymphoma

Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer

17. High Throughput RNA Sequencing in B-Cell Lymphomas

Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

Martin J.S. Dyer

8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas

Nikos Darzentas and Kostas Stamatopoulos

9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies

Sebastian B?ttcher, Matthias Ritgen, and Michael Kneba

10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR

Christiane Pott, Monika Br?ggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba

11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation

Miriam Sonnet, Constance B?r, Michael Rehli, Dieter Weichenhan, and Christoph Plass

12. Gene Expression Profile Analysis of Lymphomas

Katia Basso and Ulf Klein

13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas

Maciej Giefing and Reiner Siebert

14. Identification of P