Lysosomes: Methods and Protocols

1. SILAC-Based Comparative Proteomic Analysis of Lysosomes from Mammalian Cells Using LC-MS/MS
Melanie Thelen, Dominic Winter, Thomas Braulke, and Volkmar Gieselmann

2. Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics
Mesut Bilgin, Jesper Nylandsted, Marja J??ttel?, and Kenji Maeda

3. Analysis of N- And O-Glycosylation of Lysosomal Glycoproteins
Elmira Tokhtaeva, Olga A. Mareninova, Anna S. Gukovskaya, and Olga Vagin

4. Analyzing Lysosome Related Organelles By Electron Microscopy
Ilse Hurbain, Maryse Romao, Ptissam Bergam, Xavier Heiligenstein, And Gra?a Raposo

5. Microscopic Analysis of Lysosomal Membrane Permeabilization
Ana Maria Vilamill Giraldo, Karin ?llinger, and Vesa Loitto

6. Quantitative Co-localization and Pattern Analysis Of Endo-Lysosomal Cargo in Subcellular Image Cytometry and Validation on Synthetic Image Sets
Frederik W. Lund and Daniel W?stner

7. Preparation of a Two-Photon Fluorescent Probe for Imaging H2O2 in Lysosomes in Living Cells and Tissues
Mingguang Ren, Beibei Deng, Xiuqi Kong, Yonghe Tang, and Weiying Lin

8. Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy Dependent Recovery
Takanobu Otomo and Tamotsu Yoshimori

9. Delivery of Cargo to Lysosomes using Gneosomes
Kristina M. Hamill, Ezequiel Wexselblatt, Wenyong Tong, Jeffrey D. Esko, and Yitzhak Tor

10. Lysosomal Acidification in Cultured Astrocytes Using Nanoparticles
Camilla L??v and Anna Erlandsson

11. Analysis of Lysosomal pH by Flow Cytometry Using FITC-dextran Loaded Cells
Ida Eriksson, Karin ?llinger, and Hanna Appelqvist

12. Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry
Anna L. S?dergren and Sofia Ramstr?m

13. Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes
Norma W. Andrews

14. Using the MEROPS Database for Investigation of Lysosomal Peptidases, their Inhibitors and Substrates
Neil D. Rawlings

15. Next Generation Sequencing Approaches to Define the Role of the Autophagy Lysosomal Pathway in Human Disease: The Example of Lysoplex
Giuseppina Di Fruscio, Sandro Banfi, Vincenzo Nigro, and Andrea Ballabio

16. Gelatin Zymography using Leupeptin for the Detection of Various Cathepsin L Forms
Yoko Hashimoto

17. Methods for Determination of Α-Glycosidase, Β-Glycosidase, and Α-Galactosidase Activities in Dried Blood Spot Samples
Eser Yıldırım Sozmen and Ebru Demirel Sezer

18. Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic Villi
Jyotsna Vermaa, Sunita Bijarnia-Mahaya, and Ishwar C. Vermaa

19. Lysosomal Biology in Cancer
Colin Fennelly and Ravi Amaravadi