Maize: Methods and Protocols

Preface...
Table of Contents...
Contributing Authors...

Part I Cell, Tissue, and Organ Culture and Maize Transformation

1 Use of In Vitro Kernel Culture to Study Maize Nitrogen and Carbohydrate Metabolism
Juliann R. Seebauer and Frederick E. Below

2 Agrobacterium- and Biolistic-Mediated Transformation of Maize B104 Inbred
Jennifer A. Raji, Bronwyn Frame, Daniel Little, Tri Joko Santoso, and Kan Wang

3 Advances in Agrobacterium-Mediated Maize Transformation
Heng Zhong, Sivamani Elumalai, Samson Nalapalli, Lee Richbourg, Anna Prairie, David Bradley, Shujie Dong, Jenny Su, Weining Gu, Tim Strebe, Liang Shi, and Qiudeng Que

4 A Brief History of Promoter Development for Use in Transgenic Maize Applications
Part II Gene Silencing and Generation of Mutant Populations

5 Deletion Mutagenesis and Identification of Causative Mutations in Maize
Shangang Jia, Aixia Li, Chi Zhang, and David Holding

6 Selective Knockdowns in Maize by Sequence-Specific Protein Aggregation
Camilla Betti, Joost Schymkowitz, Frederic Rousseau, and Eugenia Russinova

7 Virus-Induced Gene Silencing in Maize with a Foxtail mosaic virus Vector
Yu Mei and Steven A. Whitham

8 Use of a Virus Gene Silencing Vector for Maize Functional Genomics Research
Tao Zhou, Xuedong Liu, and Zaifeng Fan

Part III Plant Gene Expression

9 Laser-Capture Microdissection of Maize Kernel Compartments for RNA-Seq-Based Expression Analysis
Shanshan Zhang, Dhiraj Thakare, and Ramin Yadegari

10 Ribosome Profiling in Maize
Prakitchai Chotewutmontri, Nicholas Stiffler, Kenneth P. Watkins, and Alice Barkan

11 Detection of DNA Methylation by Whole-Genome Bisulfite Sequencing
Qing Li, Peter J. Hermanson, and Nathan M. Springer

12 Plant Gene Regulation Using Multiplex CRISPR-dCas9 Artificial Transcription Factors
Levi G. Lowder, Aimee Malzahn, and Yiping Qi

13 Cellular and Sub-Cellular Level Localization of Maize Lipids and Metabolites Using High-Spatial Resolution MALDI Mass Spectrometry Imaging
Maria Emilia Due?as, Adam D. Feenstra, Andrew R. Korte, Paige Hinners, and Young Jin Lee