Metalloproteins: Methods and Protocols

Part I. Metalloproteins

1. Nitrogenases

Nate Sickerman, Yilin Hu, and Markus W. Ribbe

2. Bacteriochlorophyll and Coenzyme F430

J?rgen Moser and Gunhild Layer

3. Carbon Monoxide Dehydrogenases

Jae-Hun Jeoung, Berta M. Martins, and Holger Dobbek

4. Molybdenum-Containing Enzymes

Dimitri Niks and Russ Hille

5. Hydrogenases

Nathaniel S. Sickerman and Yilin Hu

Part II. Genetic and Biochemical Methods

6. Genomic Manipulations of the Diazotroph Azotobacter vinelandii

Patricia Dos-Santos

7. Purification of Nitrogenase Proteins

Chi Chung Lee, Markus W. Ribbe, and Yilin Hu

8. Expression, Purification and Activity Analysis of Chlorophyllide Oxidoreductase and Ni2+-Sirohydrochlorin a, c-Diamide Reductase

J?rgen Moser, Jan Jasper, Jos? Vazquez Ramos, Sven T. Sowa, and Gunhild Layer

9. Reconstitution of Molybdoenzymes with Bis-Molybdopterin Guanine Dinucleotide Cofactor

Paul Kaufmann, Chantal Iobbi-Nivol, and Silke Leimk?hler

Part III. Structural and Spectroscopic Methods

10. Crystallization of Nitrogenase Proteins

Belinda B. Wenke, Renee J. Arias, and Thomas Spatzal

11. X-Ray Crystallography of Carbon Monoxide Dehydrogenases

12. X-Ray Absorption Spectroscopy of Metalloproteins

13. Electron Paramagnetic Resonance Spectroscopy of Metalloproteins

Andrew Jasniewski, Yilin Hu, and Markus Ribbe

14. Magnetic Circular Dichroism Spectroscopy of Metalloproteins

Brian J. Hales

Part IV. Chemical methods and Theoretical calculations

15. Chemical Synthesis of an Asymmetric Mimic of the Nitrogenase Active Site

Kazuki Tanifuji and Yasuhiro Ohki

16. Computational Methods for Modeling Metalloproteins

Martin Stiebritz and Yilin Hu