Platelets and Megakaryocytes: Volume 3, Additional Protocols and Perspectives

Part I: Additional Protocols for the Study of Platelet Function

1. Intravital Imaging of Thrombus Formation in Small and Large Mouse Arteries: Experimentally-Induced Vascular Damage and Plaque Rupture In Vivo

Marijke J.E. Kuijpers and Johan W.M. Heemskerk

2. Assessment of Platelet Aggregation Responses In Vivo in the Mouse

Christopher Moore and Michael Emerson

3. Focal Cerebral Ischemia

Stefan Braeuninger, Christoph Kleinschnitz, Bernhard Nieswandt, and Guido Stoll

4. Extracorporeal Assays of Thrombosis

Lina Badimon, Teresa Padro, and Gemma Vilahur

5. Platelet Life Span and Apoptosis

Emma C. Josefsson, Michael J. White, Mark R. Dowling, and Benjamin T. Kile

6. A Live Cell Micro-Imaging Technique to Examine Platelet Calcium Signalling Dynamics Under Blood Flow

Warwick S. Nesbitt, Ian S. Harper, Simone M. Schoenwaelder, Yuping Yuan, and Shaun P. Jackson

7. Platelet Shape Change and Spreading

Joseph E. Aslan, Asako Itakura, Jacqueline M. Gertz, and Owen J.T. McCarty

8. Clot Retraction

Katherine L. Tucker, Tanya Sage, and Jonathan M. Gibbins

9. Visualization and Manipulation of the Platelet and Megakaryocyte Cytoskeleton

Jonathan N. Thon and Joseph E. Italiano

10. Measurement of Platelet Microparticles

Jeffrey I. Zwicker, Romaric Lacroix, Fran?oise Dignat-George, Barbara C. Furie, and Bruce Furie

11. Assessing Protein Synthesis by Platelets

Hansj?rg Schwertz, Jesse W. Rowley, Neal D. Tolley, Robert A. Campbell, and Andrew S. Weyrich

12. A Rapid and Efficient Platelet Purification Protocol for Platelet Gene Expression Studies

Stefan Amisten

Part II: Additional Protocols for the Study of Megakaryocyte Function

13. Characterization of Megakaryocyte Development in the Native Bone Marrow Environment

Anita Eckly, Catherine Strassel, Jean-Pierre Cazenave, Fran?ois Lanza, Catherine L?on, and Christian Gachet

14. Culture of Murine Megakaryocytes and Platelets from Fetal Liver and Bone Marrow

Harald Schulze

15. In Vitro Generation of Megakaryocytes and Platelets from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

Naoya Takayama and Koji Eto

16. Megakaryocyte and Platelet Production from Human Cord Blood Stem Cells

Am?lie Robert, Val?rie Cortin, Alain Garnier, and Nicolas Pineault

17. Culture of Megakaryocytes and Platelets from Subcutaneous Adipose Tissue and a Preadipocyte Cell Line

Yumiko Matsubara, Mitsuru Murata, and Yasuo Ikeda

18. Purification of Native Bone Marrow Megakaryocytes for Studies of Gene Expression

Gwen Tolhurst, Richard N. Carter, Nigel Miller, and Martyn P. Mahaut-Smith

19. Assessment of Megakaryocyte Migration and Chemotaxis

Alexandra Mazharian

Part III: Perspectives on the Study of Platelet and Megakaryocyte Function

20. Megakaryopoiesis and Thrombopoiesis: An Update on Cytokines and Lineage Surface Markers

Ming Yu and Alan B. Cantor

21. Using Zebrafish (Danio rerio) to Assess Gene Function in Thrombus Formation

Christopher M. Williams and Alastair W.