Molecular Toxicology Protocols

Part I. Toxicoproteomics

1. Array-Based Immunoassays with Rolling-Circle Amplification Detection

Katie Partyka, ShuangshuangWang, Ping Zhao, Brian Cao, and Brian Haab

2. Analysis of Protein Changes Using Two Dimensional Difference Gel Electrophoresis

Weimin Gao

Part II. Toxicant-Induced Inflammation

3. Assessment of Pathological and Physiological Changes in Mouse Lung Through Bronchoalveolar Lavage

Yuanpu Peter Di

4. Analysis of Clinical and Biological Samples Using Microsphere-Based Multiplexing Luminex System

Yingze Zhang, Rahel Birru, and Y. Peter Di

Part III. Gene Promoter Methylation

5. Detection of DNA Methylation by MeDIP and MBDCap Assays: An Overview of Techniques

Hang-Kai Hsu, Yu-I Weng, Pei-Yin Hsu, Tim H.-M. Huang, and Yi-Wen Huang

6. Screening of DNA Methylation Changes by Methylation-Sensitive Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR)

Kamaleshwar P. Singh

Part IV. Array Technologies

7. Strategies for Measurement of Biotransformation Enzyme Gene Expression

Marjorie Romkes and Shama C. Buch

8. Genotyping Technologies: Application to Biotransformation Enzyme Genetic Polymorphism Screening

Marjorie Romkes and Shama C. Buch

9. TaqMan Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Material

Kaori Shintani-Ishida, Bao-Li Zhu, and Hitoshi Maeda

Part V. Analysis of DNA Adducts

10. 32P-Postlabelling Analysis of DNA Adducts

David H. Phillips and Volker M. Arlt

11. Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot

Masako Ochiai, Takashi Sugimura, and Minako Nagao

12. DNA Isolation and Sample Preparation for Quantitation of Adduct Levels by Accelerator Mass Spectrometry

Karen H. Dingley, Esther A. Ubick, John S. Vogel, Ted J. Ognibene, Michael A. Malfatti, Kristen Kulp, and Kurt W. Haack

13. Analysis of DNA Strand Cleavage at Abasic Sites

Walter A. Deutsch and Vijay Hegde

Part VI. Detection of Chromosomal and Genome-Wide Damage

14. Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes without Mitogen Stimulation for Chromosome Aberration Analysis Using Specific Whole Chromosome DNA Hybridization Probes

Rupak Pathak and Pataje G. S. Prasanna

15. Mutagen Sensitivity as Measured by Induced Chromatid Breakage as a Marker of Cancer Risk

Xifeng Wu, Yun-Ling Zheng, and T. C. Hsu

16. Pulsed-Field Gel Electrophoresis Analysis of Multicellular DNA Double-Strand Break Damage and Repair

Nina Joshi and Stephen G. Grant

Part VII. Detection and Characterization of Surrogate Gene Mutation

17. Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice

Vasily N. Dobrovolsky, Xuefei Cao, Javed A. Bhalli, and Robert H. Heflich

18. The Blood-Vased Glycophorin A Human In vivo Somatic Mutation Assay

Nicole T. Myers and Stephen G. Grant

19. Flow Cytometric Quantification of Mutant T Cells with Altered Expression of the T-Cell Receptor: Detecting Somatic Mutants in Humans and Mice

Seishi Kyoizumi, Yoichiro Kasunoki, and Tomonori Hayashi

20. Analysis of in Vivo Mutation at the HPRT and TK Genes of Mouse Lymphocytes

Vasily N. Dobrovolsky, Joseph G. Shaddock, and Robert H. Heflich

21. Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems

Roy R. Swiger

22. The Human T-Cell Cloning Assay: Ide