Mouse Models of Innate Immunity: Methods and Protocols

1. Conventional Murine Gene Targeting

Albert G. Zimmermann and Yue Sun

2. Production and Characterization of Humanized Rag2-/-γc-/- Mice

Freddy M. Sanchez, German I. Cuadra, Stanton J. Nielsen, Anne Tanner, and Bradford K. Berges

3. Isolation, Culture, and Functional Evaluation of Bone Marrow-Derived Macrophages

Beckley K. Davis

4. Collecting Resident or Thioglycollate Elicited Peritoneal Macrophages

Monika Schneider

5. Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils

Linda Vong, Philip M Sherman, and Michael Glogauer

6. Assessment of Oxidative Metabolism

Emilie Imbeault and Denis Gris

7. Generation and Culture of Mouse Embryonic Fibroblasts

Yu Lei

8. Primary Ear Fibroblast Derivation from Mice

Chris B. Moore and Irving C. Allen

9. Bone Marrow Derived Dendritic Cells

Kelly Roney

10. Measuring T Cell Function in Innate Immune Models

Brianne R. Barker

11. Bioassay for the Measurement of Type-I Interferon Activity

Douglas G. Widman

12. Safe and Effective Mouse Foot-Pad Inoculation

Kristin M. Long and Mark Heise

13. Delayed Type Hypersensitivity Models in Mice

Irving C. Allen

14. Mouse Model of Staphylococcus aureus Skin Infection

Natalia Malachowa, Scott D. Kobayashi, Kevin R. Braughton, and Frank R. DeLeo

15. Sepsis Induced by Cecal Ligation and Puncture

Haitao Wen

16. Systemic Infection of Mice with Listeria monocytogenes to Characterize Host Immune Responses

Nancy Wang, Richard A. Strugnell, Odilia L. Wijburg, and Thomas C. Brodnicki

17. Mouse Model of Invasive Fungal Infection

Donna M. MacCallum

18. Endotoxin-Induced Uveitis in Rodents

Umesh C.S. Yadav and Kota V. Ramana

19. Bacteria-Mediated Acute Lung Inflammation

Irving C. Allen

20. Intranasal Influenza Infection of Mice and Methods to Evaluate Progression and Outcome

Catherine J. Sanders, Brian Johnson, Charles W. Frevert, and Paul G. Thomas

21. Dextran Sodium Sulfate-Induced Murine Inflammatory Colitis Model

Monika Schneider

22. Bacterial-Mediated Gastrointestinal Inflammation

Joshua Uronis and Xiaolun Sun

23. Plasmodium berghei ANKA (PbA) Infection of C57BL/6J Mice: A Model of Severe Malaria

Marcela Montes de Oca and Ashraful Haque

24. Characterization of HIV-1 Infection in the Humanized Rag2-/-γc-/- Mouse Model

Freddy M. Sanchez and Bradford K. Berges