Mtor: Methods and Protocols
(Book #821 in the Methods in Molecular Biology Series)
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1. Mammalian Target of Rapamycin: A Signaling Kinase for every Aspect of Cellular Life
Thomas Weichhart
2. Biochemical and Pharmacological Inhibition of mTOR by Rapamycin and an ATP-Competitive mTOR Inhibitor
Ker Yu and Lourdes Toral-Barza
3. Evaluation of the Nutrient-Sensing mTOR Pathway
Sungki Hong, Aristotle M. Mannan and Ken Inoki
4. mTOR Activity Under Hypoxia
Douangsone D. Vadysirisack and Leif W. Ellisen
5. Isolation of the mTOR Complexes by Affinity Purification
Tattym Shaikenov, Olga Bulgakava, Rakhment I. Bersimbaev and Dos D. Sarbassov
6. An in vitro Assay for the Kinase Activity of mTOR Complex 2
Jingxiang Huang
7. Overexpression or Downregulation of mTOR in Mammalian Cells
Mahmoud Khalil and Ivan Gout
8. Dectection of Cytoplasmic and Nuclear Functions of mTOR by Fractionation
Margit Rosner and Markus Hengstschl?ger
9. Evaluation of Rapamycin-Induced Cell Death
Lorenzo Galluzzi, Eugenia Morselli, Oliver Kepp, Ilio Vitale, Am?na Ben Younes, Maria Chiara Maiuri and Guido Kroemer
10. Evaluation of mTOR-Regulated mRNA Translation
Valentina Iadevaia, Xuemin Wang, Zhong Yao, Leonard J. Foster and Christopher G. Proud
11. A Genome-wide RNAi Screen for Polypeptides that Alter rpS6 Phosphorylation
Angela Papageorgiou and Josep
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