Myogenesis: Methods and Protocols

1. Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS)

Anna Pakula, Janelle M. Spinazzola, and Emanuela Gussoni

2. Electrical Pulse Stimulation of Primary Human Skeletal Muscle Cells

Natasa Nikolic and Vigdis Aas

3. Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD

Jingjuan Chen, Chao Wang, and Shihuan Kuang

4. Chromatin Immunoprecipitation in Skeletal Myoblasts

Vinay Kumar Rao, Shilpa Rani Shankar, and Reshma Taneja

5. Exercising Bioengineered Skeletal Muscle In Vitro: Biopsy to Bioreactor

Daniel C. Turner, Andreas M. Kasper, Robert A. Seaborne, Alexander D. Brown, Graeme L. Close, Mark Murphy, Claire E. Stewart, Neil R.W. Martin, and Adam P. Sharples

6. Isolation and Purification of Satellite Cells from Young Rats by Percoll Density Gradient Centrifugation

7. Lentivirus-Mediated RNAi in Skeletal Myogenesis

Dongwook Kim, Adriana Reyes-Ordo?ez, and Jie Chen

8. Adipogenesis from Bovine Precursors

Frea Mehta, Ruud Theunissen, and Mark J. Post

9. Transcriptomic Profiling during Myogenesis

Alicja Majewska, Tomasz Domoradzki, and Katarzyna Grzelkowska-Kowalczyk

10. Co-Culture Method to Obtain Endothelial Networks within Human Tissue-Engineered Skeletal Muscle

Dacha Gholobova, Melanie Gerard, Lisanne Terrie, Linda Desender, Janet Shansky, Herman Vandenburgh, and Lieven Thorrez

11. Interaction between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System

12. LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue

Marta Moreno-Torres, Jesper F. Havelund, and Nils J. Faergeman

13. siRNA Gene Silencing during C2C12 Myogenesis

Alexis Parent?, Luce P?ri?, Laetitia Magnol, Khaled Bouhouche, and V?ronique Blanquet

Dhanushika Ratnayake and Peter D. Currie

15. Preparation of Proliferated Bovine Primary Skeletal Muscle Cells for Bottom-Up Proteomics by LC-MSMS Analysis

Eva Veiseth-Kent, Vibeke H st, and Mona E. Pedersen

16. Myogenesis in Drosophila melanogaster: Dissection of Distinct Muscle Types for Molecular Analysis