Nanotechnology for Nucleic Acid Delivery: Methods and Protocols

1. Sequence-Defined Cationic Lipo-Oligomers Containing Unsaturated Fatty Acids for Transfection

S?ren Reinhard and Ernst Wagner

2. Synthesis of Bioreducible Polycations with Controlled Topologies

Ye-Zi You, Jun-Jie Yan, Fei Yu, Zhi-Qiang Yu, and David Oupick?

3. Histidine-Rich Cationic Cell Penetrating Peptides for Plasmid DNA and siRNA Delivery

Antoine Kichler, A. James Mason, Arnaud Marquette, and Burkhard Bechinger

4. Covalent Fluorophore Labeling of Oligonucleotides and Generation of Other Oligonucleotide Bioconjugates

Cornelia Lorenzer and Johannes Winkler

5. Synthetic Oligonucleotides in SPECT/CT In Vivo Imaging: Chemical Modifications, In111 Complex Formation, Incorporation into Drug Delivery Systems

Maxim Antopolsky

6. Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids

Wolfgang R?dl, Alexander Taschauer, David Schaffert, Ernst Wagner, and Manfred Ogris

7. Cationic Photopolymerized Polydiacetylenic (PDA) Micelles for siRNA Delivery

Manon Ripoll, Patrick Neuberg, Jean-Serge Remy, and Antoine Kichler

8. Lipids for Nucleic Acid Delivery: Cationic or Neutral Lipoplexes, Synthesis, and Particle Formation

Michel Bessodes, Helene Dhotel, and Nathalie Mignet

9. Preparation, Characterization, and In Vitro Evaluation of Lipidoid-Polymer Hybrid Nanoparticles for siRNA Delivery to the Cytosol

Kaushik Thanki, Xianghui Zeng, and Camilla Foged

10. Layer-By-Layer Assembled Nanoparticles for siRNA Delivery

Michaela Guter and Miriam Breunig

11. Layer-By-Layer Film Engineering for Sequential Gene Delivery

Lingxiao Xie, Yi Zou, Sean Carroll, Maria Muniz, and Guangzhao Mao

12. Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles

Angela K. Pannier, Tyler Kozisek, and Tatiana Segura

13. In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nano-Films

Yi Zou, Lei Wan, Jenifer Blacklock, Lingxiao Xie, Sean Carroll, David Oupicky, and Guangzhao Mao

14. Lyophilization of Synthetic Gene Carriers

Julia Christina Kasper, Sarah Hedtrich, and Wolfgang Friess

15. Firefly Luciferase-Based Reporter Gene Assay for Investigating Nanoparticle-Mediated Nucleic Acid Delivery

Katharina M?ller, Manfred Ogris, and Haider Sami

16. Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles