Non-Natural Nucleic Acids: Methods and Protocols

1. Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs

Stella Diafa, Damien Ev?quoz, Christian J. Leumann, and Marcel Hollenstein

2. Synthesis of Site-Specific Crown Ether Adducts to DNA Abasic Sites, 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine and 2'-Deoxycytidine

Na An, Aaron M. Fleming, Nicole C. Rosecrans, Yi Liao, and Cynthia J. Burrows

3. Synthesis of a Fluorescent Cytidine TNA Triphosphate Analogue

Hui Mei and John Chaput

4. Synthesis of Base-Modified dNTPs through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA

Petra M?nov?, Hana Cahov?, Milan Vr?bel, and Michal Hocek

5. 2ʹ-C,4ʹ-C-Ethyleneoxy-Bridged 2ʹ-Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesis, Duplex-Forming Ability, and Enzymatic Stability

Takashi Osawa, Satoshi Obika, and Yoshiyuki Hari

6. Synthesis Protocols for Simple Uncharged Glycol Carbamate Nucleic Acids

Tanaya Bose and Vaijayanti A. Kumar

7. Synthesis of Nucleobase-Functionalized Morpholino Monomers

Bappaditya Nandi, Sankha Pattanayak, Sibasish Paul, Jayanta Kundu, and Surajit Sinha

8. Synthesis and Application of LKγT Peptide Nucleic Acids

Nathaniel Shank, Kara M. George Rosenker, Ethan A. Englund, Andrew V. Dix, Elizabeth E. Rastede, and Daniel H. Appella

9. Aminoglycoside Functionalization as a Tool for Targeting Nucleic Acids

Derrick Watkins, Krishnagopal Maiti, and Dev P. Arya

10. Preparation and Purification of Oligodeoxynucleotide Duplexes Containing a Site-Specific, Reduced, Chemically-Stable Covalent Interstrand Cross-Link between a Guanine Residue and an Abasic Site

Maryam Imani Nejad, Xu Guo, Kurt Housh, Christopher Nel, Zhiyu Yang, Nathan E. Price, Yinsheng Wang, and Kent S. Gates

11. Copper-Catalyzed Alkyne-Azide Cycloaddition on the Solid Phase for the Preparation of Fully Click-Modified Nucleic Acids

Malte Rosenthal, Franziska Pfeiffer, and G?nter Mayer

12. Labeling Peptide Nucleic Acids with Indium-111

Igor G. Panyutin

13. Site-Specific Labeling of DNA via PCR with an Expanded Genetic Alphabet

Michael P. Ledbetter, Denis A. Malyshev, and Floyd E. Romesberg

14. Flexible Nucleic Acids (FNAs) As Informational Molecules: Enzymatic Polymerization of fNTPs on DNA Templates and Nonenzymatic Oligomerization of RNA on FNA Templates

Maryline Chemama and Christopher Switzer

15. Artificial Nucleosides as Diagnostic Probes to Measure Translesion DNA Synthesis

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