Olfactory Receptors: Methods and Protocols

Part I: Knowledge Dissemination of Olfactory Receptors

1. Databases in SenseLab for the Genomics, Proteomics, and Function of Olfactory Receptors

Luis N. Marenco, Gautam Bahl, Lorra Hyland, Jing Shi, Rixin Wang, Peter C. Lai, Perry L. Miller, Gordon M. Shepherd, and Chiquito J. Crasto

2. HORDE: Comprehensive Resource for Olfactory Receptor Genomics

Tsviya Olender, Noam Nativ, and Doron Lancet

3. Identification of Olfactory Receptor Genes from Mammalian Genome Sequences

Yoshihito Niimura

Part II: Theoretical Assessments of Olfactory Receptor Structure and Function

4. Molecular Modelling of Odorant / Olfactory Receptor Complexes

Landry Charlier, J?r?mie Topin, Peter C. Lai, Chiquito J. Crasto, and Jerome Golebiowski

5. The Electrical Properties of Olfactory Receptors in the Development of Biological Smell Sensors

Eleanora Alfinito, Jeremy Pousset, and Lino Reggiani

6. A Hit Map-Based Statistical Method to Predict Best Ligands for Orphan Olfactory Receptors: Natural Key Odorants Versus 'Lock Picks'

Dietmar Krautwurst and Matthias Kotthoff

Part III: Expression Systems for Olfactory Receptors

7. A Robust, Rapid, and Simple Method of Producing Olfactory Receptors Using Commercial E. coli Cell-Free Systems

Karolina Corin, Brian Cook, and Shuguang Zhang

8. Human Olfactory Receptors: Recombinant Expression in the Baculovirus / Sf9 Insect Cell System, Functional Characterization, and Odorant Identification

Val?ry Matarazzo and Catherine Ronin

9. Single Cell RT-PCR Identification of Odorant Receptors Expressed by Olfactory Neurons

Bettina Malnic

10. Next-Generation Sequencing of the Human Olfactory Receptors

Joel D. Mainland, Jason R. Willer, Hiroaki Matsunami, and Nicholas Katsanis

11. Deciphering Activation of Olfactory Receptors Using Heterologous Expression in Saccharomyces cerevisiae and Bioluminescence Resonance Energy Transfer (BRET)

Guenha?l Sanz and Edith Pajot-Augy

12. Olfactory Receptors in Human Airway Epithelia

Xiaoling Gu and Yehuda Ben-Shahar

Part IV: Functional Analysis of Olfactory Receptors

13. Functional Characterization of Insect Olfactory Receptor Neurons Through In Vivo Approaches

Michel Renou and Philippe Lucas

14. Functional Assay of Mammalian and Insect Olfactory Receptors Using Xenopus Oocytes

Charles W. Luetje, Andrew S. Nichols, Ana Castro, and Benjamin L. Sherman

15. Pharmacology of Mammalian Olfactory Receptors

Richard S. Smith, Zita Peterlin, and Ricardo C. Araneda

16. Visualizing Olfactory Receptor Expression and Localization in Drosophila

Michael Saina and Richard Benton

17. Deorphanization of Human Olfactory Receptors by Luciferase and Ca-Imaging Methods

Kaveh A. Baghaei

Part V: Olfactory Receptor Evolution

18. Evolution of Olfactory Receptors

Kara C. Hoover