Optogenetics: Methods and Protocols

1. Optogenetics: Basic Concepts and Their Development
Yong Ku Cho and Dan Li

2. Natural Resources for Optogenetic Tools
Tilo Mathes

3. Algal Photobiology: A Rich Source of Unusual Light Sensitive Proteins for Synthetic Biology and Optogenetics
Arash Kianianmomeni and Armin Hallmann

4. Reversible Photoregulation of gene Expression and Translation
Shinzi Ogasawara

5. Controlling Protein Activity and Degradation Using Blue-Light
Anne P. Lutz, Christian Renicke, and Christof Taxis

6. Photo-Control of Protein Function Using Photoactive Yellow Protein
Jakeb M. Reis and G. Andrews Woolley

7. A Fluorometric Activity Assay for Light-Regulated Cyclic-Nucleotide-Monophosphate Actuators
Charlotte Helene Chumacher, Heinz G. K?rschen, Christopher Nicol, Carlos Gasser, Reinhard Seifert, Martin Schw?rzel, and Andreas M?glich

8. Optogenetic Control of Pancreatic Islets
Thomas M. Reinbothe and In?s G. Mollet

9. Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression
Rocio Ochoa-Fernandez, Sophia L. Samodelov, Simon M. Brandl, Elke Wehinger, Konrad M?ller, Wilfried Weber, and Matias D. Zurbriggen

10. Perfect Channelrhodopsins: A User Guide
Jonas Wietek and Matthias Prigge

11. Optogenetics in Drosophila Neuroscience
Thomas Riemensperger, Robert J. Kittel, and Andr? Fiala

12. Optogenetic Control of Mammalian Ion Channels with Chemical Photoswitches
Damien Lemoine, Romain Durand-de Cuttoli, and Alexandre Mourot

13. Optogenetic Modulation of Locomotor Activity on Free Behaving Rats
Kedi Xu, Jiacheng Zhang, Songchao Guo, and Xiaoxiang Zheng

14. Combines Optogenetic and Chemogenetic Control of Neurons
Ken Berglund, Jack K. Tung, Bryan Higashikubo, Robert E. Gross, Christopher I. Moore, and Ute Hochgeschwender
15. Intracranial Injection of an Optogenetics Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in Rat Brain Cortex
Christopher Pawela, Edgar DeYoe, and Ramin Pashaie

16. An Optomized Calcium-Phosphate Transfection Method for Characterizing Genetically Encoded Tools in Primary Neurons
Shiyao Wang and Yong Ku Cho

17. Optogenetic Approaches for Mesoscopic Brain Mapping
Michael Kyweriga and Majid H. Mohajerani

18. Optogenetic Tools for Confined Stimulation in Deep Brain Structures
Alexandre Castonguay, S?bastien Thomas, Fr?d?ric Lesage, and Christian Casanova

19. Remote Patterning of Transgene Expression Using Near Infrared-Responsive Plasmonic Hydrogels
Francisco Mart?n-Saavedra and Nuria Vilaboa

20. Optogenetic Light Crafting Tools for the Control of Cardiac Arrhythmias
Claudia Richter, Jan Christoph, Stephan E. Lehnart, and Stefan Luther

21. Inscribing Optocal Excitability to Non-Excitable Cardiac Cells: Viral Delivery of Optogenetic Tools in Primary Cardiac Fibroblasts