Plant Cell Culture Protocols

1. An Introduction to Plant Cell Culture

Victor M. Loyola-Vargas and Neftal? Ochoa-Alejo

2. History of Plant Tissue Culture

Trevor Thorpe

3. Callus, Suspension Culture and Hairy Roots. Induction, Maintenance, and Characterization

Rosa M. Gal?z-?valos, Sagrario Aguilar-D?az, Pedro A. Xool-Gonz?lez, Silvia M. Huch?n-May and V?ctor M. Loyola-Vargas

4. Growth Measurements. Estimation of Cell Division and Cell Expansion

Gregorio Godoy-Hern?ndez and Felipe A. V?zquez Flota

5. Measurement of Cell Viability

Lizbeth A. Castro-Concha, Rosa Mar?a Escobedo, and Mar?a de Lourdes Miranda-Ham

6. Pathogen and Biological Contamination Management in Plant Tissue Culture: Phyto-Pathogens, Vitro-Pathogens, and Vitro-Pests

Alan C. Cassells

7. Cryopreservation of Embryogenic Cell Suspensions by Encapsulation-Vitrification and Encapsulation-Dehydration

Zhenfang Yin, Long Chen, Bing Zhao, Yongxing Zhu, and Qiaochun Wang

8. The Study of in vitro Development in Plants: General Approaches and Photography

Edward C. Yeung

9. Use of Statistics in Plant Biotechnology

Michael E. Compton

10. Tissue Culture Methods for the Clonal Propagation and Genetic Improvement of Spanish Red Cedar (Cedrela Odorata)

Yuri Pe?a-Rodr?guez, Juan Ju?rez G?mez, Jos? Antonio Gonz?lez, and Manuel L. Robert

11. Micropropagation of Banana

Yıldız Aka Ka?ar and Ben Faber

12. Liquid in vitro Culture for the Propagation of Arundo donax

Miguel ?ngel Herrera-Alamillo and Manuel L. Robert

13. Production of Haploids and Doubled Haploids in Maize

Vanessa Prigge and Albrecht E. Melchinger

14. Maize Somatic Embryogenesis: Recent Features to Improve Plant Regeneration

Ver?nica Garrocho-Villegas, Maria Teresa de Jes?s-Olivera, and Estela S?nchez Quintanar

15. Improved Shoot Regeneration from Root Explants Using an Abscisic Acid Containing Medium

Subramanian Paulraj and Edward C. Yeung

16. Cryopreservation of Shoot Tips and Meristems: An Overview of Contemporary Methodologies

Erica E. Benson and Keith Harding

17. Anther Culture of Chili Pepper (Capsicum spp.)

Neftal? Ochoa-Alejo

18. Production of Inter-Specific Hybrids in Ornamental Plants

Juntaro Kato and Masahiro Mii

19. Plant Tissue Culture of Fast-Growing Trees for Phytoremediation Research

Jos? Luis Couselo, Elena Corredoira, Ana M. Vieitez, and Antonio Ballester

20. Removing Heavy Metals by in vitro Cultures

Mar?a del Socorro Santos-D?az and Mar?a del Carmen Barr?n-Cruz

21. Establishment of a Sanguinarine Producing Cell Suspension Culture of Argemone Mexicana L. (Papaveraceae). Induction of Alkaloid Accumulation

Felipe A. V?zquez-Flota, Miriam Monforte-Gonz?lez, Cecilia Gu?zar-Gonz?lez, Jorge Rubio-Pi?a, and Karen Trujillo-Villanueva

22. Epigenetics, the Role of DNA Methylation on Tree Development

Marcos Viejo Somoano, Mar?a E. Santamar?a, Jos? L. Rodr?guez, Luis Valledor, M?nica Meij?n, Marta P?rez, Jes?s Pascual, Rodrigo Hasb?n, Mario Fern?ndez Fraga, Mar?a Berdasco, Peter E. Toorop, Mar?a J. Ca?al, and Roberto Rodr?guez Fern?ndez

23. The Potential Roles of microRNAs in Molecular Breeding

Qing Liu and Yue-Qin Chen

24. Determination of Histone Methylation in M