Polyamines: Methods and Protocols

Part I: Introduction

1. Current Status of the Polyamine Research Field

Anthony E. Pegg and Robert A. Casero, Jr.

Part II: Identification of Polyamine Metabolic Enzymes and Characterization of Polyamine-Regulated Genes and Ion Channels

2. Exploring Polyamine Biosynthetic Diversity through Comparative and Functional Genomics

Anthony J. Michael

3. Characterization of Genes for Polyamine Modulon

Kazuei Igarashi and Keiko Kashiwagi

4. Posttranscriptional Regulation of Gene Expression in Epithelial Cells by Polyamines

Lan Xiao and Jian-Ying Wang

5. Identification, Chemical Synthesis, and Biological Functions of Unusual Polyamines Produced by Extreme Thermophiles

Tairo Oshima, Toshiyuki Moriya, and Yusuke Terui

6. Polyamine Block of Inwardly-Rectifying Potassium Channels

Harley T. Kurata, Wayland W.L. Cheng, and Colin G. Nichols

Part III: Transgenic Models for Study of Polyamine Function

7. Carcinogenesis Studies in Mice with Genetically Engineered Alterations in Polyamine Metabolism

David J. Feith

8. Transgenic Rodents with Altered SSAT Expression as Models of Pancreatitis and Altered Glucose and Lipid Metabolism

Anne Uimari, Mervi T. Hyv?nen, Eija Pirinen, and Leena Alhonen

9. Use of (Gyro) Gy and Spermine Synthase Transgenic Mice to Study Functions of Spermine

Xiaojing Wang and Anthony E. Pegg

Part IV: Enzymes Involved in Polyamine Catabolism and Post-Translational Protein Modification

10. A Simple Assay for Mammalian Spermine Oxidase: A Polyamine Catabolic Enzyme Implicated in Drug Response and Disease

Andrew C. Goodwin, Tracy R. Murray-Stewart, and Robert A. Casero, Jr.

11. Characterization, Assay, and Substrate Specificity of Plant Polyamine Oxidases

Panagiotis N. Moschou and Kalliopi A. Roubelakis-Angelakis

12. Assay of Deoxyhypusine Synthase Activity

Edith C. Wolff, Seung Bum Lee, and Myung Hee Park

13. Assay of Deoxyhypusine Hydroxylase Activity

Jong Hwan Park, Edith C. Wolff, and Myung Hee Park

Part V: Regulation of Polyamine Content

14. Identification and Assay of Allosteric Regulators of S-Adenosylmethionine Decarboxylase

Erin K. Willert, Lisa N. Kinch, and Margaret A. Phillips

15. Protocols for Studying Antizyme Expression and Function

Noriyuki Murai, Yasuko Murakami, and Senya Matsufuji

16. Identification, Assay, and Functional Analysis of the Antizyme Inhibitor Family

Chaim Kahana

17. Post-Transcriptional Regulation of Ornithine Decarboxylase

Shannon L. Nowotarski, Sofia Origanti, and Lisa M. Shantz

Part VI: Polyamine Transport and Uptake

18. Identification and Assays of Polyamine Transport Systems in Escherichia coli and Saccharomyces cerevisiae

Keiko Kashiwagi and Kazuei Igarashi

19. Genetic and Biochemical Analysis of Protozoal Polyamine Transporters

Marie-Pierre Hasne and Buddy Ullman

20. Heparan Sulfate Proteoglycan-Mediated Polyamine Uptake

Johanna Welch, Katrin Svensson, Paulina Kucharzewska, and Mattias Belting