Here is a step-by-step guide to the general methods used for protein research. For each method, all reagents and items of equipment are described; a detailed protocol is presented; and potential problems, and pitfalls are discussed. Basic methods for extracting, handling, storing and measuring the concentration of proteins are presented. Various approaches to the study of proteins, using gel electrophoresis and related methods, are also covered. It will allow a novice to perform the various procedures. Experienced research workers will find critical information concerning advanced procedures assembled in a single source for the first time.
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