Quorum Sensing: Methods and Protocols
(Book #1673 in the Methods in Molecular Biology Series)
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Book Overview
Part I: Detection and Quantification of Quorum Sensing Signal Molecules
1. Use of Whole Cell Bioassays for Screening Quorum Signaling, Quorum-Interference, and Biofilm Dispersion
Starla G. Thornhill and Robert J.C. McLean
2. Detection of 2-Alkyl-4-Quinolones Using Biosensors
Matthew P. Fletcher, Stephen P. Diggle, Miguel C?mara, and Paul Williams
3. "Hot Stuff" The Many Uses of a Radiolabel Assay in Detecting Acyl-Homoserine Lactone Quorum Sensing Signals
Amy L. Schaefer, Caroline S. Harwood, and E. Peter Greenberg
4. Liquid Chromatography/Mass Spectrometry (LC/MS) for the Detection and Quantification of -Acyl-L-Homoserine Lactones (AHLs) and 4-Hydroxy-2-Alkylquinolines (HAQs)
Fran?ois L?pine, Sylvain Milot, Marie-Christine Groleau, and Eric D?ziel
5. Detection of the Bacterial Quorum Sensing Signaling Molecules N-Acyl Homoserine Lactones (HSL) and N-Acyl-Homoserine (HS) with an Enzyme-Linked Immunosorbent Assay (ELISA) and via Ultrahigh Performance Liquid Chromatography Coupled to Mass Spectrometry (UHPLC-MS)
Michael Rothballer, Jenny Uhl, Josie Kunze, Philippe Schmitt-Kopplin, and Anton Hartmann
6. Biosensors for the Detection and Quantification of AI-2 Class Quorum Sensing Compounds
Sathish Rajamani and Richard Sayre
7. Detection of Agr-Type Autoinducing Peptides Produced by Staphylococcus aureus
Ewan J. Murray and Paul Williams
8. Ultra Performance Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of Diffusible Signal Factor (DSF)-Family Quorum Sensing Signals
Lian Zhou, Shuang Sun, Wei Zhang, and Ya-Wen He
9. Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode
Alyah Buzid, John H.T. Luong, F. Jerry Reen, Fergal O'Gara, Jeremy D. Glennon, and Gerard P. McGlacken
10. Detection and Quantification of Butyrolactones from Streptomyces
Marc Biarnes-Carrera, Rainer Breitling, and Eriko Takano
Part II: Methods for the Studying of Quorum Sensing at the Molecular, Physiological, and Population Level
11. Fluorescence Quenching Studies of γ-Butyrolactone Binding Protein (CprB) from Streptomyces coelicolor A3(2)
Jessy Mariam and Ruchi Anand
12. Methods to Study Solo/Orphan Quorum Sensing Receptors
Vittorio Venturi, Sujatha Subramoni, Anice Sabag-Daigle, and Brian M.M. Ahmer
13. Enzymatic Assays to Investigate Acyl-Homoserine Lactone Autoinducer Synthases
Daniel Shin and Rajesh Nagarajan
14. Global Expr
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