Receptor Binding Techniques

1. Receptor Databases and Computational Websites for Ligand Binding

Brinda K. Rana, Philip E. Bourne and Paul A. Insel

2. How to use the IUPHAR Receptor Database to Navigate Pharmacological Data

Chidochangu P. Mpamhanga, Joanna L. Sharman, Anthony J. Harmar, and NC-IUPHAR

3. Radioligand Binding Assays and their Analysis

Janet J. Maguire, Rhoda E. Kuc and Anthony P. Davenport

4. Use of Scintillation Proximity Assay (SPA) to Measure Radioligand Binding to Immobilised Receptors without Separation of Bound from Free Ligand

Jenny Berry, Molly Price-Jones, and Barbara Killian

5. Visualisation and Analysis of Vascular Receptors using Confocal Laser Scanning Microscopy and Fluorescent Ligands

Craig J Daly, Ingela Parmryd and John C. McGrath

6. Dissecting the Pharmacology of G Protein Coupled Receptor Signalling Complexes using Bimolecular Fluorescence Complementation

Laura E Kilpatrick and Nicholas D Holliday

7. Live Cell Imaging of G Protein-Coupled Receptors

Anke Teichmann, Antje Schmidt , Burkhard Wiesner , Alexander Oksche , and Ralf Sch?lein

8. Characterization of G-protein Coupled Receptor (GPCR) Modulators using Homogeneous cAMP Assays

Daniel L. Bassoni, Qumber Jafri, Sunitha Sastry, Mahesh Mathrubutham and Tom Wehrman

9. Measurements of β-arrestin Recruitment to Activated Seven Transmembrane Receptors using Enzyme Complementation

Daniel L. Bassoni, William J. Raab, Philip L. Achacoso, Chin Yee Loh and Tom S. Wehrman

10. Quantitative Phosphor Imaging Autoradiography of Radioligands for Positron Emission Tomography

Peter Johnstr?m, Joseph L. Bird and Anthony P. Davenport

11. Dynamic In Vivo Imaging of Receptors in Small Animals using Positron Emission Tomography

Peter Johnstr?m, Tim D. Fryer, Joseph L. E. Bird, Hugh K. Richards, and Anthony P. Davenport

12. Cellular Localization of Receptors using Antibodies Visualised by Light and Dual Labelling Confocal Microscopy

Anthony P. Davenport and Rhoda E. Kuc

13. Detection of mRNA Encoding Receptors by in Situ And Northern Hybridisation

Alessandra P. Princivalle, Rachel M.C Parker, Terri J. Dover, and Nicholas M. Barnes