Rnai and Small Regulatory Rnas in Stem Cells: Methods and Protocols

Part I: Introduction

1. RNAi Technique in Stem Cell Research: Current Status and Future Perspectives

Gang-Ming Zou

2. RNAi and MicroRNA-Mediated Gene Regulation in Stem Cells

Alexander K. Murashov

Part II: RNA Interference

3. Forward RNAi Screens in Human Hematopoietic Stem Cells

Roman Galeev, Christine Karlsson, Aur?lie Baudet, and Jonas Larsson

4. Silencing of ATP11B by RNAi-Induced Changes in Neural Stem Cell Morphology

Jiao Wang, Qian Wang, Fangfang Zhou, Dong Wang, and Tieqiao Wen

5. High-Efficiency Transfection of Glioblastoma Cells and a Simple Spheroid Migration Assay

6. Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in Embryonic Stem Cells

Xiaoxing Cheng and Rui Jian

7. Efficient Depletion of Essential Gene Products for Loss-of-Function Studies in Embryonic Stem Cells

Soizik Berlivet, Isabelle Hmitou, H?l?ne Picaud, and Matthieu G?rard

8. Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAi Directed Against Transcription Factor L3/Lhx8

Takayuki Manabe and Akio Wanaka

9. A Recessive Genetic Screen for Components of the RNA Interference Pathway Performed in Mouse Embryonic Stem Cells

Melanie I. Trombly and Xiaozhong Wang

10. RNAi Knockdown of Ape1 Gene in the Differentiation of Mouse Embryonic Stem Cells

Gang-Ming Zou, Jieqing Yu, Cynthia LeBron, and Yumei Fu

11. An Efficient Transfection Method for Differentiation and Cell Proliferation of Mouse Embryonic Stem Cells

Jun-Yang Liou, Bor-Sheng Ko, and Tzu-Ching Chang

12. Implanting Glioblastoma Spheroids into Rat Brains and Monitoring Tumor Growth by MRI Volumetry

Mario L?hr, Thomas Linsenmann, Anna Jawork, Almuth F. Kessler, Nils Timmermann, Gy?rgy A. Homola, Ralf-Ingo Ernestus, and Carsten Hagemann

Amelia J. Helms, Danielle S. Finger, Victoria L. Hardy, and Elizabeth T. Ables

14. In Vivo RNAi in the Drosophila Follicular Epithelium: Analysis of Stem Cell Maintenance, Proliferation, and Differentiation

Veit Riechmann

15. A Phenotype-Based RNAi Screening for Ras-ERK/MAPK Signaling-Associated Stem Cell Regulators in C. elegans

Myon-Hee Lee and Dong Suk Yoon

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