Schwann Cells: Methods and Protocols

PART I: Protocols for preparing primary cultures, cell lines and engineered Schwann cells

1. Isolation of Schwann Cell Precursors from Rodents
Rhona Mirsky and Kristjan R. Jessen

2. Preparation of Neonatal Rat Schwann cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies

Patrice Maurel

3. Isolation and Expansion of Schwann Cells from Transgenic Mouse Models
Jihyun Kim and Haesun Kim

4. Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers

Natalia D. Andersen and Paula V. Monje

5. Detailed Protocols for the Isolation, Culture, Enrichment, and Immunostaining of Primary Human Schwann cells

Tamara Weiss, Sabine Taschner-Mandl, Peter F. Ambros, and Inge M. Ambros

6. Magnetic-activated Cell Sorting for the Fast and Efficient Separation of Human and Rodent Schwann Cells from Mixed Cell Populations

Kristine M. Ravelo, Natalia D. Andersen, and Paula V. Monje

7. Culture and Expansion of Rodent and Porcine Schwann Cell for Pre-clinical Animal Studies

Adriana E. Brooks, Gagani Athauda, Mary Bartlett Bunge, and Aisha Khan

8. Chemical Conversion of Human Fibroblasts into functional Schwann Cells

Eva C. Thoma

9. Derivation of Fate-committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats

Y.P. Tsui, Graham K. Shea, Y.S. Chan, and Daisy K. Shum

10. Human Induced Pluripotent Stem Cell (hiPS cell)-derived Sensory Neurons for Fate Commitment of Bone Marrow Stromal Cell-derived Schwann Cells

Sa Cai, Daisy KY Shum, and Ying-Shing Chan

11. Generation and Use of Merlin-Deficient Human Schwann Cells for a High-Throughput Chemical Genomics Screening Assay
Alejandra M. Petrilli and Cristina Fern?ndez-Valle

PART II: Protocols for studying Schwann cells behavior and myelination in vitro

12. Lentiviral Transduction of Rat Schwann Cells and Dorsal Root Ganglia Neurons for In Vitro Myelination Studies

Corey Heffernan and Patrice Maurel

13. Preservation, Sectioning and Staining of Schwann Cell Cultures for Transmission Electron Microscopy Analysis

Vania W. Almeida, Margaret L. Bates, and Mary Bartlett Bunge

14. Scalable Differentiation and Dedifferentiation Assays using Neuron-free Schwann Cell Cultures

Paula V. Monje

15. The Pseudopod System for Axon-glia Interactions: Stimulation and Isolation of Schwann cell Protrusions that form in Response to Axonal Membranes

Yannick Poitelon and M. Laura Feltri

16. In vitro Analysis of the Role of Schwann Cells on Axonal Degeneration and Regeneration using Sensory Neurons from Dorsal Root Ganglia

Rodrigo L?pez-Leal, Paula Diaz, and Felipe A. Court

17. A Culture Model To Study Neuron - Schwann cell - Astrocyte Interactions

Susana R. Cerqueira, Yee-Shuan Lee, and Mary Bartlett Bunge

18. Preparation of Matrices of Variable Stiffness for the Study of Mechanotransduction in Schwann Cell Development
Mateusz M. Urbanski and Carmen V. Melendez-Vasquez

19. Purification of Exosomes from Primary Schwann cells, RNA Extraction and Next Generation Sequencing of Exosomal RNAs

Cristian De Gregorio, Paula D?az, Rodrigo L?pez-Leal, Patricio Manque, and Felipe A. Court

20. 3D Cancer Migration Assay with Schwann Cells

Laura Fangmann, Steffen Teller, Pavel Stupakov, Helmut Friess, G?ralp O. Ceyhan, and Ihsan Ekin Demir

PART III: Protocols for studying Schwann cell development and myelination in viv