Single-Cell Analysis: Methods and Protocols

1. Introduction: Why Analyze Single Cells?

Dino Di Carlo, Henry Tat Kwong Tse, and Daniel R. Gossett

Part I: Conventional Methods

2. Flow Cytometry and Microscopy as Means of Studying Single Cells: A Short Introductional Overview

Sara Lindstr?m

3. Identification of Enzyme-Converted Peptide Products from Single Cells Using Capillary Electrophoresis and Liquid Chromatography-Mass Spectrometry

Robert B. Brown, Johannes A. Hewel, Andrew Emili, and Julie Audet

4. Laser Capture Microdissection for Gene Expression Analysis of Specific Cell Populations in Single Blastocysts

Ward De Spiegelaere, Muriel Filliers, and Ann Van Soom

Part II: Miniaturized Solutions

5. Single-Cell Culture in Microwells

Sara Lindstr?m and Helene Andersson-Svahn

6. Iso-Dielectric Separation and Analysis of Cells

Michael D. Vahey and Joel Voldman

7. Single Cell Electroporation Using Microfluidic Devices

S?verine Le Gac and Albert van den Berg

8. Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Build-In Pillar Array

Chi Zhang

9. Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in the Single-Cell Comet Assay

Sara Henriksson and Mats Nilsson

10. Droplet Microfluidics for Single-Cell Analysis

Eric Brouzes

11. Screening of Antigen-Specific Antibody Secreting Cells

Hiroyuki Kishi, Aishun Jin, Tatsuhiko Ozawa, Kazuto Tajiri, Tsutomu Obata, and Atsushi Muraguchi

12. Analysis of Single Eukaryotic Cells Using Raman Tweezers

Elsa Correia Faria and Peter Gardner

13. Single-Cell Microinjection Technologies

Yan Zhang

14. Ultrasonic Manipulation of Single Cells

Martin Wiklund and Bj?rn ?nfelt

Part III: Reviews on Chosen Subjects Within the Field

15. Expanding the Horizons for Single-Cell Applications on Lab-On-a-Chip Devices

Soo Hyeon Kim, Dominique Fourmy, and Teruo Fujii

16. Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses

Ay?a Yal?ın, Yvonne J. Yamanaka, and J. Christopher Love