Sphingosine-1-Phosphate: Methods and Protocols

1. Biochemical Methods for Quantifying Sphingolipids: Ceramide, Sphingosine, Sphingosine Kinase-1 Activity, and Sphingosine-1-Phosphate

Brizuela Leyre and Cuvillier Olivier

2. Isoform-Selective Assays for Sphingosine Kinase Activity

Melissa R. Pitman, Duyen H. Pham, and Stuart M. Pitson

3. Quantification of Sphingosine-1-Phosphate and Related Sphingolipids by Liquid Chromatography Coupled to Tandem Mass Spectrometry

Constantin Bode and Markus H. Gr?ler

4. A Clean-up Method for Mass Spectrometric Analysis of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule

Jun-ichi Morishige, Tamotsu Tanaka, and Kiyoshi Satouchi

5. Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples

Barbara Visentin, Gary Reynolds, and Roger Sabbadini

6. Assessment of Sphingosine-1-Phosphate Activity in Biological Samples by Receptor Internalization and Adherens Junction Formation

Hideru Obinata and Timothy Hla

7. High Throughput Assays to Measure Intracellular Ca2+ Mobilization in Cells that Express Recombinant S1P Receptor Subtypes

William J. Valentine and Gabor Tigyi

8. Quantifying Sphingosine-1-Phosphate-Dependent Activation of the RhoGTPases

Matt D. Medlin, Joan M. Taylor, and Christopher P. Mack

9. Optimized Protocols to Analyze Sphingosine-1-Phosphate Signal Transduction Pathways During Acrosomal Exocytosis in Human Sperm

Silvia A. Belmonte and Laila Suhaiman

10. Use of Intravital Microscopy and In Vitro Chemotaxis Assays to Study the Roles of Sphingosine-1-Phosphate in Bone Homeostasis

Taeko Ishii, Shunsuke Kawamura, Issei Nishiyama, Junichi Kikuta, and Masaru Ishii

11. Assessment of Sphingosine-1-Phosphate Receptor Expression and Associated Intracellular Signaling Cascades in Primary Cells of the Human Central Nervous System

Veronique E. Miron, Bryce A. Durafourt, Jack P. Antel, and Timothy E. Kennedy

12. Sphingosine-1-Phosphate Signaling in Skeletal Muscle Cells

Elisabetta Meacci, Francesca Bini, and Chiara Battistini

13. Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor

Raymond C.B. Wong, Martin F. Pera, and Alice P?bay

14. Ceramide and Sphingosine-1-Phosphate Signaling in Embryonic Stem Cell Differentiation

Erhard Bieberich

15. Sphingosine-1-Phosphate: An Alternative to Serum for Culture and Multipotency Maintenance of Adult Human Mesenchymal Stem Cells

He Xiaoli, Sathish Jean, and Alirio J. Melendez

16. Sphingosine-1-Phosphate Signaling in Neural Progenitors

Phillip Callihan and Shelley B. Hooks

17. Utilizing Sphingosine-1-Phosphate to Stimulate Sprouting Angiogenesis

Shih-Chi Su and Kayla J. Bayless