Stem Cell Banking: Concepts and Protocols

Preface...
Table of Contents...
Contributing Authors...

Part I Generic Themes in Stem Cell Banking

1. Stem Cell Banking: A Global ViewGlyn Stacey
2. Quality Assurance in Stem Cell Banking: Emphasis on Embryonic and Induced Pluripotent Stem Cell BankingTher?se Kallur, Pontus Blomberg, Sonya Stenfelt, Kristian Tryggvason, and Outi Hovatta
3. Acquisition and Reception of Primary Tissues, Cells, or Other Biological SpecimensLyn E. Healy
4. Information ManagementAlberto Labarga, Izaskun Beloqui, and Angel G. Martin
5. Cryopreservation: Vitrification and Control Rate CoolingCharles J. Hunt
6. Quality Assured Characterization of Stem Cells for Safety in Banking for Clinical ApplicationKevin Bruce, John D.M. Campbell, and Paul De Sousa
7. Ethics and Governance of Stem Cell BanksDon Chalmers, Peter Rathjen, Joy Rathjen, and Dianne Nicol
Part II Protocols for Pluripotent Stem Cell Banking
8. Derivation of Human Embryonic Stem CellsJeremy M. Crook, Lucy Kravets, Teija Peura, and Meri T. Firpo
9. Derivation of Human Induced Pluripotent Stem Cells in Chemically Defined MediumGuokai Chen and Mahendra Rao
10. Culture, Adaptation, and Expansion of Pluripotent Stem CellsJennifer L. Brehm and Tenneille E. Ludwig
11. Cryobanking Pluripotent Stem CellsJeremy M. Crook, Eva Tomaskovic-Crook, and Tenneille E. Ludwig
12. Genome Editing in Human Pluripotent Stem CellsJared Carlson-Stevermer and Krishanu Saha

Part III Protocols for Mesenchymal Stem Cell Banking
13. Isolation, Culture, and Expansion of Mesenchymal Stem CellsIzaskun Ferrin, Izaskun Beloqui, Lorea Zabaleta, Juan M. Salcedo, Cesar Trigueros, and Angel G. Martin
14. Cryobanking Mesenchymal Stem CellsAndr?s Pav?n, Izaskun Beloqui, Juan M. Salcedo, and Angel G. Martin
Part IV Protocols for Human Neural Stem Cell Banking
15. Culturing and Cryobanking Human Neural Stem CellsJeremy M. Crook and Eva Tomaskovic-Crook