Stem Cells and Aging: Methods and Protocols

1. Comprehensive Hematopoietic Stem Cell Isolation Methods

Kyle Rector, Yi Liu, and Gary Van Zant

2. Serial Transplantation of Bone Marrow to Test Self-Renewal Capacity of Hematopoietic Stem Cells In Vivo

Charusheila Ramkumar, Rachel M. Gerstein, and Hong Zhang

3. Mouse Hematopoietic Stem Cell Transplantation

Hui Cheng, Paulina H. Liang, and Tao Cheng

4. Isolation, Characterization, and Transplantation of Adult Liver Progenitor Cells

Mladen I. Yovchev, Mariana D. Dabeva, and Michael Oertel

5. Isolation of Muscle-Derived Stem/Progenitor Cells Based on Adhesion Characteristics to Collagen-Coated Surfaces

Mitra Lavasani, Aiping Lu, Seth D. Thompson, Paul D. Robbins, Johnny Huard, and Laura J. Niedernhofer

6. Human Myoblasts from Skeletal Muscle Biopsies: In Vitro Culture Preparations for Morphological and Cytochemical Analyses at Light and Electron Microscopy

Manuela Malatesta, Marzia Giagnacovo, Rosanna Cardani, Giovanni Meola, and Carlo Pellicciari

7. Cardiac Stem Cell Senescence

Daniela Cesselli, Federica D'Aurizio, Patrizia Marcon, Natascha Bergamin, Carlo Alberto Beltrami, and Antonio Paolo Beltrami

8. Isolation of Mesenchymal Stem Cells from Human Bone and Long-Term Cultivation Under Physiologic Oxygen Conditions

Sebastian Klepsch, Angelika Jamnig, Daniela Trimmel, Magdalena Schimke, Werner Kapferer, Regina Brunauer, Sarvpreet Singh, Stephan Reitinger, and G?nter Lepperdinger

9. Methods for Assessing Effects of Wnt/β-Catenin Signaling in Senescence of Mesenchymal Stem Cells

Hai-jie Wang and Yu-zhen Tan

10. Intra-Femoral Injection of Human Mesenchymal Stem Cells

Sindhu T Mohanty and Ilaria Bellantuono

11. Tracking of Replicative Senescence in Mesenchymal Stem Cells by Colony-Forming Unit Frequency

Anne Schellenberg, Hatim Hemeda, and Wolfgang Wagner

12. Chromatin Immunoprecipitation Coupled by Quantitative Real-Time PCR as a Tool for Analyzing Epigenetic Regulation of Stem Cell Aging

Seunghee Lee, Ji-Won Jung, and Kyung-Sun Kang

13. Quantitative Fluorescence In Situ Hybridization (Q-FISH) on Paraffine Embedded Tissue

Mario Ricciardi, Mauro Krampera, and Marco Chilosi

Daniela Cesselli, Federica D'Aurizio, Patrizia Marcon, Natascha Bergamin, Carlo Alberto Beltrami, and Antonio Paolo Beltrami

8. Isolation of Mesenchymal Stem Cells from Human Bone and Long-Term Cultivation Under Physiologic Oxygen Conditions

Sebastian Klepsch, Angelika Jamnig, Daniela Trimmel, Magdalena Schimke, Werner Kapferer, Regina Brunauer, Sarvpreet Singh, Stephan Reitinger, and G?nter Lepperdinger

9. Methods for Assessing Effects of Wnt/β-Catenin Signaling in Senescence of Mesenchymal Stem Cells

Hai-jie Wang and Yu-zhen Tan

10. Intra-Femoral Injection of Human Mesenchymal Stem Cells

Sindhu T Mohanty and Ilaria Bellantuono

11. Tracking of Replicative Senescence in Mesenchymal Stem Cells by Colony-Forming Unit Frequency

Anne Schellenberg, Hatim Hemeda, and Wolfgang Wagner

12. Chromatin Immunoprecipitation Coupled by Quantitative Real-Time PCR as a Tool for Analyzing Epigenetic Regulation of Stem Cell Aging

Seunghee Lee, Ji-Won Jung, and Kyung-Sun Kang

13. Quantitative Fluorescence In Situ Hybridization (Q-FISH) on Paraffine Embedded Tissue

Mario Ricciardi, Mauro Krampera, and Marco Chilosi

Anne Schellenberg, Hatim Hemeda, and Wolfgang Wagner

12. Chromatin Immunoprecipitation Coupled by Quantitative Real-Time PCR as a