T-Cell Trafficking: Methods and Protocols

1. Introduction to Homeostatic Migration

Mark C. Coles

2. Analysis of Thymocyte Migration, Cellular Interactions, and Activation by Multi-Photon Fluorescence Microscopy of Live Thymic Slices

Jessica N. Lancaster and Lauren I. R. Ehrlich

3. Visualising and Tracking T Cell Motility in vivo

Robert A Benson, James M Brewer, and Paul Garside

4. Graph Theory-based Analysis of the Lymph Node Fibroblastic Reticular Cell Network

Mario Novkovic, Lucas Onder, Gennady Bocharov, and Burkhard Ludewig

5. Visualizing Endogenous Effector T Cell Egress from the Lymph Nodes

Manisha Menon, Alexandre P Benechet, and Kamal M Khanna

6. Introduction: T cell Trafficking in Inflammation and Immunity

Myriam Chimen, Bonita H. R. Apta, and Helen M. McGettrick

7. Leukocyte Adhesion under Haemodynamic Flow Conditions

Charlotte Lawson, Marlene Rose, and Sabine Wolf

Bonita H. R. Apta, Myriam Chimen, and Helen M. McGettrick

9. Mesenchymal Stromal Cells as Active Regulators of Lymphocyte Recruitment to Blood Vascular Endothelial Cells

10. Monitoring RhoGTPase Activity in Leukocytes using Classic "pull-down" Assays

Marouan Zarrouk, David Killock, Izajur Rahman, Jessica Davies, and Aleksandar Ivetic

11. Utilising Lentiviral Gene Transfer in Primary Endothelial Cells to Assess Lymphocyte-Endothelial Interactions

Jasmeet S. Reyat, Michael G. Tomlinson, and Peter J. Noy

12. Introduction to Lymphocyte Trafficking in Disease

Patricia F. Lalor and Elizabeth Hepburn

13. Using Ex-Vivo Liver Organ Cultures to Measure Lymphocyte Trafficking

Ben Wiggins, Zania Stamataki, and Patricia F. Lalor

14. In vitro and Ex Vivo Models to Study T Cell Migration through the Human Liver Parenchyma

Benjamin G. Wiggins, Konstantinos Aliazis, and Scott P. Davies

15. Monitoring Migration of Activated T Cells to Antigen-rich Non-lymphoid Tissue

Eleanor Jayne Ward, Hongmei Fu, and Federica Marelli Berg

16. Tissue Digestion for Stromal Cell and Leukocyte Isolation

Saba Nayar, Joana Campos, Nathalie Steinthal, and Francesca Barone

17. T-cell Response in the Lung Following Influenza Virus Infection

Robert A. Benson, Jennifer C. Lawton, and Megan K.L. MacLeod