Teratogenicity Testing: Methods and Protocols

Part I: Introduction to Teratology Testing

1. An Overview of Teratology

Ana M. Calado and Maria dos Anjos Pires

2. Teratology Study Guidelines: An Overview

Sofia Alves-Pimenta, Lu?s F?lix, Bruno Cola?o, Paula A. Oliveira, and Carlos Ven?ncio

3. Biological Concerns on the Selection of Animal Models for Teratogenic Testing

Sofia Alves-Pimenta, Bruno Cola?o, Paula A. Oliveira, and Carlos Ven?ncio

Part II: In Vitro-Based Teratology Screening

4. The Validated Embryonic Stem Cell Test with Murine Embryonic Stem Cells

Lauren M. Walker, Darcie L.V. McClelland-Descalzo, and Nicole I. zur Nieden

5. Human Pluripotent Stem Cells to Assess Developmental Toxicity in the Osteogenic Lineage

Joseph V. Madrid, Steven R. Sera, Nicole R.L. Sparks, and Nicole I. zur Nieden

6. Chick Embryonic Cardiomyocytes Micromass System for Assessing Developmental Cardiotoxicity of Drugs

Wasay M.S. Qureshi and Margaret K. Pratten

7. Flow Cytometry to Evaluate Potential Developmental Toxicants in the Embryonic Stem Cell

Val?ria Maria Lara, Kelly C. Santos Roballo, Andr? Tadeu Gotardo, and Carlos Eduardo Ambr?sio

8. Morphology-Based Whole Embryo Culture for Developmental Toxicity of Drugs

Bo Liang and Chi C. Wang

9. Western Blot Methodologies for Analysis of In Vitro Protein Expression Induced by Teratogenic Agents

Carlos Martins-Gomes and Am?lia M. Silva

10. Manipulation of MicroRNAs in Cultured Mouse Embryos: Applications for Developmental Toxicology

Fuqiang Yuan and Shao-yu Chen

Part III: Animal-Based Teratology Methods

11. Insights into the Phenotypic and Behavioral Effects of Teratogenic Drugs in Caenorhabditis elegans

Caralina M. de Evsikova

12. Effect of Teratogens on Development of Drosophila melanogaster

Dong Li and Xiaolin Bi

13. Cellular Responses in Drosophila melanogaster Following Teratogen Exposure

Matheus C. Bianchini, Jos? L.R. Portela, Robson L. Puntel, and Daiana S. ?vila

14. Behavioral Teratogenesis in Drosophila melanogaster

Monalisa Mishra and Bedanta Kumar Barik

15. Evaluation of the Teratogenicity of Pharmaceuticals Using FETAX

Hariz Islas-Flores, Itzayana P?rez-Alvar?z, and Leobardo M. G?mez-Oliv?n

16. Histological Observation of Teratogenic Phenotypes Induced in Frog Embryo Assays

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