The Plant Cell Wall: Methods and Protocols

1. Plant Tissue Cultures

Anna K?rk?nen, Arja Santanen, Kuninori Iwamoto, and Hiroo Fukuda

2. Computerized Molecular Modelling of Carbohydrates

Alfred D. French and Glenn P. Johnson

3. Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS

Markus G?nl, Florian Kraemer, and Markus Pauly

4. High-Voltage Paper Electrophoresis (HVPE) of Cell-Wall Building Blocks and Their Metabolic Precursors

Stephen C. Fry

5. Carbohydrate Gel Electrophoresis

Florence Goubet, Paul Dupree, and Katja Salomon Johansen

6. Capillary Electrophoresis with Detection by Laser-Induced Fluorescence

Andrew Mort and Xiangmei Wu

7. Monoclonal antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Plant Cell Walls

C?cile Herv?, Susan E. Marcus, and J. Paul Knox

8. Screening and Characterization of Plant Cell Walls Using Carbohydrate Microarrays

Iben S rensen and William G.T. Willats

9. Electron Tomography and Immunogold Labelling as Tools to Analyze de novo Assembly of Plant Cell Walls

Marisa S. Otegui

10. Analyzing Cellulose Biosynthesis with Confocal Microscopy

Meera Nair and Seth DeBolt

11. Visual Mapping of Cell Wall Biosynthesis

Yumiko Sakuragi, Morten H.H. N rholm, and Henrik V. Scheller

12. Atomic Force Microscopy of Plant Cell Walls

Andrew R. Kirby

13. Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organization of Primary Plant Cell Walls

Tracey J. Bootten, Philip J. Harris, Laurence D. Melton, and Roger H. Newman

14. Formation of Cellulose-Based Composites with Hemicelluloses and Pectins Using Gluconacetobacter Fermentation

Deirdre Mikkelsen and Michael J. Gidley

15. Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis

Derek T.A. Lamport, Li Tan, and Marcia J. Kieliszewski

16. New Insights into the Control of Cell Growth

Claudia Blaukopf, Matth?us Z. Krol, and Georg J. Seifert

17. Extraction and Detection of Arabinogalactan Proteins

Zo? A. Popper

18. Characterization of the Plant Cell Wall Proteome Using High Throughput Screens

Sang-Jik Lee and Jocelyn K.C. Rose

19. Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens

Alison W. Roberts, Christos S. Dimos, Michael J. Budziszek, Jr., Chessa A. Goss, and Virginia Lai

20. Measuring In vitro Extensibility of Growing Plant Cell Walls

Daniel J. Cosgrove