Vaccine Adjuvants: Methods and Protocols
(Book #1494 in the Methods in Molecular Biology Series)
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Book Overview
1. Overview of Vaccine Adjuvants: Introduction, History, and Current Status
Ruchi R. Shah, Kimberly J. Hassett, and Luis A. Brito
2. Development of the CpG Adjuvant 1018: A Case Study
John D. Campbell
3. Syntheses of Human TLR8-Specific Small-Molecule Agonists
Mallesh Beesu, Hari Prasad Kokatla, and Sunil A. David
4. Semisynthesis of Analogues of the Saponin Immunoadjuvant QS-21
Alberto Fern?ndez-Tejada, William E. Walkowicz, Derek S. Tan, and David Y. Gin
5. QS-21 Adjuvant: Laboratory-Scale Purification Method and Formulation into Liposomes
Livia Brunner, Christophe Barnier-Quer, and Nicolas Collin
6. Purification of an Immunoadjuvant Saponin Fraction from Quillaja brasiliensis Leaves by Reverse-Phase Silica Gel Chromatography
Anna C.A. Yendo, Fernanda de Costa, Carla Kauffmann, Juliane D. Fleck, Grace Gosmann, and Arthur G. Fett-Neto
7. Biosynthetic Approaches to Squalene Production: The Case of Yeast
Martin Valachovič and Ivan Hapala
8. In Silico Adjuvant Design and Validation
Matthew N. Davies, Helene Pere, Iris Bosschem, Freddy Haesebrouck, Bram Flahou, Eric Tartour, Darren R. Flower, David F. Tough, and Jagadeesh Bayry
9. Manufacturing Methods for Liposome Adjuvants
Yvonne Perrie, Elisabeth Kastner, Swapnil Khadke, Carla B. Roces, and Peter Stone
10. Synthesis of Lymph Node-Targeting Adjuvants
Melissa C. Hanson and Darrell J. Irvine
11. Preparing an Adjuvanted Thermoresponsive Gel Formulation for Sublingual Vaccination
Manjari Lal, Jessica White, and Changcheng Zhu
12. Manufacture of Oil-in-Water Emulsion Adjuvants
Jean Haensler
13. Methods to Prepare Aluminum Salt-Adjuvanted Vaccines
Sachin G. Thakkar and Zhengrong Cui
14. Production of Adjuvant-Loaded Biodegradable Particles for Use in Cancer Vaccines
Cristina Maria de Barros, Emad Ibrahim Wafa, Khanidtha Chitphet, Kawther Ahmed, Sean M Geary, and Aliasger K. Salem
15. Lyophilization of Adjuvanted Vaccines: Methods for Formulation of a Thermostable Freeze Dried Product
Michelle Y. Chan, Timothy S. Dutill, and Ryan M. Kramer
16. Stressed Stability Techniques for Adjuvant Formulations
Manvi Hasija, Anthony Sheung, Nausheen Rahman, and Salvador F. Ausar
17. Particle Sizing of Nanoparticle Adjuvant Formulations by Dynamic Light Scattering (DLS) and Nanoparticle Tracking Analysis (NTA)
Michelle Y. Chan, Quinton M. Dowling, Sandra J. Sivananthan, and Ryan M. Kramer
18. Quantification of Multiple Components of Complex Aluminum-Based
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