Xenotransplantation: Methods and Protocols

1. Xenotransplantation: An Overview of the Field

Veronica Tisato and Emanuele Cozzi

2. Cloning and Expression Analyses of Pig Genes

Mireia Uribe-Herranz and Cristina Costa

3. Basic Analyses of Proteins of Interest for Xenotransplantation

Mireia Uribe-Herranz and Cristina Costa

4. Studies on Carbohydrate Xenoantigens

Dale Christiansen, Effie Mouhtouris, Paul A. Ramsland, and Mauro S. Sandrin

5. Xenoantibodies and Complement Activity Determinations in Pig to Primate Xenotransplantation

Nieves Dom?nech

6. Studies on Coagulation Incompatibilities for Xenotransplantation

Cristiana Bulato, Claudia Radu, Paolo Simioni

Roberta Sommaggio, Magdiel P?rez-Cruz, and Cristina Costa

8. Production of Transgenic and Knock Out Pigs by Somatic Cell Nuclear Transfer

Angelica M. Giraldo, Suyapa Ball, Kenneth R. Bondioli

9. Small Animal Models of Xenotransplantation

Hao Wang

10. Heart Xenotransplantation in Primate Models

Johannes Postrach, Andreas Bauer, Michael Schmoeckel, Bruno Reichart, and Paolo Brenner

11. Xenogeneic Lung Transplantation Models

Lars Burdorf, Agnes M. Azimzadeh and Richard N. Pierson III

12. Thymic Transplantation in Pig-to-nonhuman Primates for the Induction of Tolerance Across Xenogeneic Barriers

Kazuhiko Yamada and Joseph Scalea

13. Isolation of Porcine Pancreatic Islets for Xenotransplantation

Karin Ulrichs, Sibylle Eber, Bianca Schneiker, Sabine Gahn, Armin Strau , Vasiliy Moskalenko, Irina Chodnevskaja

14. Pig Neural Cells Derived from Foetal Mesencephalon as Cell Source for Intracerebral Xenotransplantation

Xavier L?v?que, V?ronique Nerri?re-Daguin, Isabelle Neveuand Philippe Naveilhan

15. Hepatocyte Xenotransplantation

Katia R. F. Lima-Quaresma, Andre Gustavo Bonavita, Matheus Kafuri Cytrangulo, Marcelo Alves Pinto, and Luiz Anast?cio Alves

16. In Vitro Repair Model of Focal Articular Cartilage Defects in Humans

D?az Prado SM, Fuentes-Boquete IM, Blanco FJ

17. Potential Zoonotic Infection of PERV in Xenotransplantation

Giada Mattiuzzo, Yashuhiro Takeuchi, Linda Scobie

18. Ethical and Regulatory Issues for Clinical Trials in Xenotransplantation

Jorge Guerra Gonz?lez

19. Some Ethical, Social and Legal Considerations of Xenotransplantation

Mar?a Jorqui Azofra and Carlos Mar?a Romeo Casabona